In vivo imaging of murid herpesvirus-4 infection

Milho, Ricardo, Smith, Christopher M., Marques, Sofia, Alenquer, Marta, May, Janet S., Gillet, Laurent, Gaspar, Miguel, Efstathiou, Stacey, Simas, J. Pedro and Stevenson, Philip G. (2009) In vivo imaging of murid herpesvirus-4 infection. Journal of General Virology, 90 1: 21-32. doi:10.1099/vir.0.006569-0

Author Milho, Ricardo
Smith, Christopher M.
Marques, Sofia
Alenquer, Marta
May, Janet S.
Gillet, Laurent
Gaspar, Miguel
Efstathiou, Stacey
Simas, J. Pedro
Stevenson, Philip G.
Title In vivo imaging of murid herpesvirus-4 infection
Formatted title
In vivo imaging of murid herpesvirus-4 infection
Journal name Journal of General Virology   Check publisher's open access policy
ISSN 0022-1317
Publication date 2009-01-01
Sub-type Article (original research)
DOI 10.1099/vir.0.006569-0
Volume 90
Issue 1
Start page 21
End page 32
Total pages 12
Place of publication Reading, Berks, United Kingdom
Publisher Society for General Microbiology
Language eng
Abstract Luciferase-based imaging allows a global view of microbial pathogenesis. We applied this technique to gammaherpesvirus infection by inserting a luciferase expression cassette into the genome of murine herpesvirus-4 (MuHV-4). The recombinant virus strongly expressed luciferase in lytically infected cells without significant attenuation. We used it to compare different routes of virus inoculation. After intranasal infection of anaesthetized mice, luciferase was expressed in the nose and lungs for 7–10 days and in lymphoid tissue, most consistently the superficial cervical lymph nodes, for up to 30 days. Gastrointestinal infection was not observed. Intraperitoneal infection was very different to intranasal, with strong luciferase expression in the liver, kidneys, intestines, reproductive tract and spleen, but none in the nose or lungs. The nose has not previously been identified as a site of MuHV-4 infection. After intranasal infection of non-anaesthetized mice, it was the only site of non-lymphoid luciferase expression. Nevertheless, lymphoid colonization and persistence were still established, even at low inoculation doses. In contrast, virus delivered orally was very poorly infectious. Inoculation route therefore had a major impact on pathogenesis. Low dose intranasal infection without anaesthesia seems most likely to mimic natural transmission, and may therefore be particularly informative about normal viral gene functions.
Keyword Chemokine binding protein
Epstein-Barr virus
Bacterial artificial chromosome
Peptide-loading complex
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Clinical Medical Virology Centre Publications
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