A gamma-herpesvirus glycoprotein complex manipulates actin to promote viral spread

Gill, Michael B., Edgar, Rachel, May, Janet S. and Stevenson, Philip G. (2008) A gamma-herpesvirus glycoprotein complex manipulates actin to promote viral spread. PLoS ONE, 3 3: e1808.1-e1808.12. doi:10.1371/journal.pone.0001808

Author Gill, Michael B.
Edgar, Rachel
May, Janet S.
Stevenson, Philip G.
Title A gamma-herpesvirus glycoprotein complex manipulates actin to promote viral spread
Journal name PLoS ONE   Check publisher's open access policy
ISSN 1932-6203
Publication date 2008-03-19
Year available 2008
Sub-type Article (original research)
DOI 10.1371/journal.pone.0001808
Open Access Status DOI
Volume 3
Issue 3
Start page e1808.1
End page e1808.12
Total pages 12
Place of publication San Francisco United States
Publisher Public Library of Science (PLoS)
Language eng
Abstract Viruses lack self-propulsion. To move in multi-cellular hosts they must therefore manipulate infected cells. Herpesviruses provide an archetype for many aspects of host manipulation, but only for alpha herpesviruses in is there much information about they move. Other herpesviruses are not necessarily the same. Here we show that Murine gamma-herpesvirus-68 (MHV-68). Induces the outgrowth of long, branched plasma membrane fronds to create an intercellular network for virion traffic. The fronds were actin-based and RhoA-dependent. Time-lapse imaging showed that the infected cell surface became highly motile and that virions moved on the fronds. This plasma membrane remodelling was driven by the cytoplasmic tail of gp48, a MHV-68 glycoprotein previously implicated in intercellular vital spread. The MHV-68 ORF58 was also required, but its role was simply transporting gp48 to the plasma membrane, since a gp48 mutant exported without ORF58 did not require ORF58 to form membrane fronds either. Together, gp48/ORF58 were sufficient to induce fronds in transfected cells, as were the homologous BDLF2/BMRF2 of Epstein-Barr virus. Gp48/ORF58 therefore represents a conserved module by which gamma-herpesviruses rearrange cellular actin to increase intercellular contacts and thereby promote their spread.
Keyword Multidisciplinary Sciences
Science & Technology - Other Topics
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID G0400427
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Clinical Medical Virology Centre Publications
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Citation counts: TR Web of Science Citation Count  Cited 25 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 31 times in Scopus Article | Citations
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