An internal ribosome entry site directs translation of the murine gammaherpesvirus 68 MK3 open reading frame

Coleman, Heather M., Brierley, Ian and Stevenson, Philip G. (2003) An internal ribosome entry site directs translation of the murine gammaherpesvirus 68 MK3 open reading frame. Journal of Virology, 77 24: 13093-13105. doi:10.1128/JVI.77.24.13093-13105.2003


Author Coleman, Heather M.
Brierley, Ian
Stevenson, Philip G.
Title An internal ribosome entry site directs translation of the murine gammaherpesvirus 68 MK3 open reading frame
Journal name Journal of Virology   Check publisher's open access policy
ISSN 0022-538X
1098-5514
Publication date 2003-12-01
Sub-type Article (original research)
DOI 10.1128/JVI.77.24.13093-13105.2003
Open Access Status DOI
Volume 77
Issue 24
Start page 13093
End page 13105
Total pages 13
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Formatted abstract
The gammaherpesviruses characteristically drive the proliferation of latently infected lymphocytes. The murine gammaherpesvirus 68 (MHV-68) MK3 protein contributes to this process in vivo by evading CD8+-T-cell recognition during latency, as well as during lytic infection. We analyzed some of the molecular mechanisms that control MK3 expression. No dedicated MK3 mRNA was detected. Instead, the MK3 open reading frame (ORF) was transcribed as part of a bicistronic mRNA, downstream of a previously unidentified ORF, 13M. The 13M/MK3 promoter appeared to extend approximately 1 kb 5′ of the transcription start site and included elements both dependent on and independent of the ORF50 lytic transactivator. MK3 was translated from the bicistronic transcript by virtue of an internal ribosome entry site (IRES) element. RNA structure mapping identified two stem-loops between 13M and MK3 that were sufficient for IRES activity in a bicistronic reporter plasmid and a third stem-loop just within the MK3 coding sequence, with a subtler, perhaps regulatory role. Overall, translation of the MHV-68 MK3 bore a striking resemblance to that of the Kaposi's sarcoma-associated herpesvirus vFLIP, suggesting that IRES elements are a common theme of latent gammaherpesvirus immune evasion in proliferating cells.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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