Intercellular gamma-herpesvirus dissemination involves co-ordinated intracellular membrane protein transport

May, Janet S., de Lima, Brigitte D., Colaco, Susanna and Stevenson, Philip G. (2005) Intercellular gamma-herpesvirus dissemination involves co-ordinated intracellular membrane protein transport. Traffic, 6 9: 780-793. doi:10.1111/j.1600-0854.2005.00316.x


Author May, Janet S.
de Lima, Brigitte D.
Colaco, Susanna
Stevenson, Philip G.
Title Intercellular gamma-herpesvirus dissemination involves co-ordinated intracellular membrane protein transport
Journal name Traffic   Check publisher's open access policy
ISSN 1398-9219
1600-0854
Publication date 2005-09-01
Year available 2005
Sub-type Article (original research)
DOI 10.1111/j.1600-0854.2005.00316.x
Open Access Status Not yet assessed
Volume 6
Issue 9
Start page 780
End page 793
Total pages 14
Place of publication Chichester, West Sussex, United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract
The murine gamma-herpesvirus-68 (MHV-68) ORF27 encodes gp48, a type 2 transmembrane glycoprotein that contributes to intercellular viral spread. Gp48 is expressed on the surface of infected cells but is retained intracellularly after transfection. In this study, we show that the multimembrane spanning ORF58 gene product is both necessary and sufficient for gp48 to reach the cell surface. ORF58-deficient MHV-68 expressed ORF27 in normal amounts, but retained it in the endoplasmic reticulum (ER). Transfected ORF27 also remained in ER, whereas green fluorescent protein-tagged ORF58 localized to the ER and trans-Golgi network. When ORF27 and ORF58 were co-transfected, they formed a protein complex and reached the cell surface. Surprisingly, ORF58 rather than ORF27 mediated cell binding via a small extracellular loop. The heavily glycosylated ORF27 component of the complex may, therefore, act mainly to protect this loop against antibody. The interdependent transport of ORF27 and ORF58 transport ensures that such protection is always present. 
Keyword Gamma-herpesvirus
Glycoprotein expression
Glycoprotein traffic
Immune evasion
Viral spread
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID G0400427
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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Citation counts: TR Web of Science Citation Count  Cited 20 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 19 times in Scopus Article | Citations
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