Murine gammaherpesvirus-68 ORF28 encodes a non-essential virion glycoprotein

May, Janet S., Coleman, Heather M., Boname, Jessica M. and Stevenson, Philip G. (2005) Murine gammaherpesvirus-68 ORF28 encodes a non-essential virion glycoprotein. Journal of General Virology, 86 4: 919-928. doi:10.1099/vir.0.80661-0

Author May, Janet S.
Coleman, Heather M.
Boname, Jessica M.
Stevenson, Philip G.
Title Murine gammaherpesvirus-68 ORF28 encodes a non-essential virion glycoprotein
Journal name Journal of General Virology   Check publisher's open access policy
ISSN 0022-1317
Publication date 2005-04-01
Year available 2005
Sub-type Article (original research)
DOI 10.1099/vir.0.80661-0
Open Access Status Not yet assessed
Volume 86
Issue 4
Start page 919
End page 928
Total pages 10
Place of publication London,United Kingdom
Publisher The Microbiology Society
Language eng
Formatted abstract
Murine gammaherpesvirus-68 (MHV-68) ORF28 is a gammaherpesvirus-specific gene of unknown function. Analysis of epitope-tagged ORF28 protein indicated that it was membrane-associated and incorporated into virions in N-glycosylated, O-glycosylated and unglycosylated forms. The extensive glycosylation of the small ORF28 extracellular domain - most forms of the protein appeared to be mainly carbohydrate by weight - suggested that a major function of ORF28 is to attach a variety of glycans to the virion surface. MHV-68 lacking ORF28 showed normal lytic replication in vitro and in vivo and normal latency establishment. MHV-68 ORF28 therefore encodes a small, membrane-bound and extensively glycosylated virion protein, whose function is entirely dispensable for normal, single-cycle host colonization.
Keyword Biotechnology & Applied Microbiology
Biotechnology & Applied Microbiology
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID G9800943
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
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Citation counts: TR Web of Science Citation Count  Cited 26 times in Thomson Reuters Web of Science Article | Citations
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