High LDL levels are associated with increased lipoprotein-associated phospholipase A2 activity on nitric oxide synthesis and reactive oxygen species formation in human endothelial cells

Searle, Andrea, Gomez-Rosso, Leonardo, Merono, Tomás, Salomon, Carlos, Duran-Sandoval, Daniel, Giunta, Gustavo, Grant, Carlos, Calvo, Carlos, Lamperti, Liliana, Brites, Fernando and Aguayo, Claudio (2011) High LDL levels are associated with increased lipoprotein-associated phospholipase A2 activity on nitric oxide synthesis and reactive oxygen species formation in human endothelial cells. Clinical Biochemistry, 44 2-3: 171-177. doi:10.1016/j.clinbiochem.2010.10.004


Author Searle, Andrea
Gomez-Rosso, Leonardo
Merono, Tomás
Salomon, Carlos
Duran-Sandoval, Daniel
Giunta, Gustavo
Grant, Carlos
Calvo, Carlos
Lamperti, Liliana
Brites, Fernando
Aguayo, Claudio
Title High LDL levels are associated with increased lipoprotein-associated phospholipase A2 activity on nitric oxide synthesis and reactive oxygen species formation in human endothelial cells
Journal name Clinical Biochemistry   Check publisher's open access policy
ISSN 0009-9120
1873-2933
Publication date 2011-01-01
Sub-type Article (original research)
DOI 10.1016/j.clinbiochem.2010.10.004
Volume 44
Issue 2-3
Start page 171
End page 177
Total pages 7
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Language eng
Formatted abstract
Objective: To evaluate in vitro the effects of serum and LDL fractions isolated from hypercholesterolemic patients on nitric oxide (NO) synthesis and reactive oxygen species (ROS) production by human umbilical vein endothelial cells (HUVECs).

Design and methods: Serum and LDL isolated from subjects with high (n=18) and normal (n=21) LDL-cholesterol levels were analyzed on NO synthesis and ROS production in vitro models of HUVECs. LDL was furthers characterized in their chemical composition and activities of lipoprotein-associated phospholipase A2 (Lp-PLA2), cholesteryl ester transfer protein (CETP) and paraoxonase.

Results: NO bioavailability was significantly lower and ROS production higher in HUVECs incubated with serum samples from patients with high LDL-cholesterol levels in comparison to control subjects. Moreover, hypercholesterolemic patients presented higher CETP and Lp-PLA2 activities than control subjects. LDL fractions isolated from patients and controls were not different in their chemical composition, Lp-PLA2 activity, and their capacity to reduce NO synthesis and increase ROS production.

Conclusion: Alterations of serum from hypercholesterolemic patients could be due to the increment in LDL concentration, main Lp-PLA2 carrier, and not to LDL composition or intrinsic Lp-PLA2 activity. 
Keyword Endothelial cells
Hypercholesterolemia
Nitric oxide
Reactive oxygen species
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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