The development and validation of an antibody-ELISA to detect Trypanosoma evansi infection in cattle in Australia and Papua New Guinea

Reid S.A. and Copeman D.B. (2003) The development and validation of an antibody-ELISA to detect Trypanosoma evansi infection in cattle in Australia and Papua New Guinea. Preventive Veterinary Medicine, 61 3: 195-208. doi:10.1016/j.prevetmed.2003.07.004


Author Reid S.A.
Copeman D.B.
Title The development and validation of an antibody-ELISA to detect Trypanosoma evansi infection in cattle in Australia and Papua New Guinea
Journal name Preventive Veterinary Medicine   Check publisher's open access policy
ISSN 0167-5877
Publication date 2003-11-12
Year available 2003
Sub-type Article (original research)
DOI 10.1016/j.prevetmed.2003.07.004
Volume 61
Issue 3
Start page 195
End page 208
Total pages 14
Publisher ELSEVIER SCIENCE BV
Language eng
Subject 1103 Clinical Sciences
3400 Veterinary
Abstract Trypanosoma evansi is exotic to Australia and Papua New Guinea (PNG). However, it might have been introduced to Papua (Indonesia); thus, there is a risk of it entering PNG and thence Australia. Because of logistical difficulties in PNG and northern Australia, surveillance for T. evansi must rely on serological tests. The accuracy of an Ab-ELISA using a detergent extract of T. evansi and three antigen fractions purified from the detergent extract using stepwise precipitation with saturated ammonium sulphate (AS) were compared. The ELISA using the AS 40-50% fraction had greater discriminatory power compared to the ELISA using the other antigen fractions. This ELISA then was compared with two commercial tests: the Card Agglutination Test for trypanosomiasis/T. evansi (CATT) and Suratex®. CATT/T. evansi at 1/4 serum dilution has higher sensitivity and the ELISA has higher specificity. There is no likely benefit in combining antibody detection tests to improve the accuracy of diagnosis. Furthermore, the combination of Suratex® (which was independent of the antibody tests) with the CATT or the ELISA did not improve the sensitivity. None of the tests was sufficiently sensitive to be used confidently to determine freedom from infection in animals imported into Australia from countries where T. evansi infection is endemic.
Keyword CATT/T. evansi
Cattle
Diagnosis
ELISA
Serology
Suratex
Trypanosoma evansi
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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