In vitro analysis of the TAT protein transduction domain as a drug delivery vehicle in protozoan parasites

Lee, Stephanie Hui Chin, Jefferies, Ryan, Watt, Paul, Hopkins, Richard, Sotzik, Frank, Reid, Simon, Armson, Anthony, Boxell, Annika and Ryan, Una (2008) In vitro analysis of the TAT protein transduction domain as a drug delivery vehicle in protozoan parasites. Experimental Parasitology, 118 3: 303-307. doi:10.1016/j.exppara.2007.08.019


Author Lee, Stephanie Hui Chin
Jefferies, Ryan
Watt, Paul
Hopkins, Richard
Sotzik, Frank
Reid, Simon
Armson, Anthony
Boxell, Annika
Ryan, Una
Title In vitro analysis of the TAT protein transduction domain as a drug delivery vehicle in protozoan parasites
Journal name Experimental Parasitology   Check publisher's open access policy
ISSN 0014-4894
1090-2449
Publication date 2008-01-01
Year available 2008
Sub-type Article (original research)
DOI 10.1016/j.exppara.2007.08.019
Volume 118
Issue 3
Start page 303
End page 307
Total pages 5
Place of publication Maryland Heights, MO United States
Publisher Academic Press
Language eng
Subject 2403 Immunology
2405 Parasitology
2725 Infectious Diseases
Abstract The present study was undertaken to analyse the capability of HIV-1 derived TAT protein transduction domain (PTD) fused with Green Fluorescent Protein (TAT-GFP) as a delivery vehicle into a range of protozoan parasites. Successful transduction of native purified TAT-GFP was observed by fluorescent microscopy in Cryptosporidium parvum, Giardia duodenalis, and Neospora caninum. The ability to transduce peptides and other cargo into protozoan parasites, will greatly assist in the delivery of future peptide-based drugs and target validation peptides.
Keyword Cryptosporidium
Drug delivery vehicle
Giardia
Neospora
Protein transduction domain (PTD)
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Public Health Publications
 
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