Predicting the dynamics of protein abundance

Mehdi, Ahmed M., Patrick, Ralph, Bailey, Timothy L. and Boden, Mikael (2014) Predicting the dynamics of protein abundance. Molecular and Cellular Proteomics, 13 5: 1330-1340. doi:10.1074/mcp.M113.033076

Author Mehdi, Ahmed M.
Patrick, Ralph
Bailey, Timothy L.
Boden, Mikael
Title Predicting the dynamics of protein abundance
Journal name Molecular and Cellular Proteomics   Check publisher's open access policy
ISSN 1535-9484
Publication date 2014-05-01
Year available 2014
Sub-type Article (original research)
DOI 10.1074/mcp.M113.033076
Volume 13
Issue 5
Start page 1330
End page 1340
Total pages 11
Place of publication Bethesda MD, United States
Publisher American Society for Biochemistry and Molecular Biology
Language eng
Formatted abstract
Protein synthesis is finely regulated across all organisms, from bacteria to humans, and its integrity underpins many important processes. Emerging evidence suggests that the dynamic range of protein abundance is greater than that observed at the transcript level. Technological breakthroughs now mean that sequencing-based measurement of mRNA levels is routine, but protocols for measuring protein abundance remain both complex and expensive. This paper introduces a Bayesian network that integrates transcriptomic and proteomic data to predict protein abundance and to model the effects of its determinants. We aim to use this model to follow a molecular response over time, from condition-specific data, in order to understand adaptation during processes such as the cell cycle. With microarray data now available for many conditions, the general utility of a protein abundance predictor is broad.

Whereas most quantitative proteomics studies have focused on higher organisms, we developed a predictive model of protein abundance for both Saccharomyces cerevisiae and Schizosaccharomyces pombe to explore the latitude at the protein level. Our predictor primarily relies on mRNA level, mRNA-protein interaction, mRNA folding energy and half-life, and tRNA adaptation. The combination of key features, allowing for the low certainty and uneven coverage of experimental observations, gives comparativelyminor but robust prediction accuracy. The model substantially improved the analysis of protein regulation during the cell cycle: predicted protein abundance identified twice as many cell-cycle-associated proteins as experimental mRNA levels. Predicted protein abundance was more dynamic than observed mRNA expression, agreeing with experimental protein abundance from a human cell line. We illustrate how the same model can be used to predict the folding energy of mRNA when protein abundance is available, lending credence to the emerging view that mRNA folding affects translation efficiency. The software and data used in this research are available at http://bioinf.scmb.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

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