Sperm protamine deficiency correlates with sperm DNA damage in Bos indicus bulls

Fortes, M. R. S., Satake, N., Corbet, D. H., Corbet, N. J., Burns, B. M., Moore, S. S. and Boe-Hansen, G. B. (2014) Sperm protamine deficiency correlates with sperm DNA damage in Bos indicus bulls. Andrology, 2 3: 370-378. doi:10.1111/j.2047-2927.2014.00196.x


 
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Author Fortes, M. R. S.
Satake, N.
Corbet, D. H.
Corbet, N. J.
Burns, B. M.
Moore, S. S.
Boe-Hansen, G. B.
Title Sperm protamine deficiency correlates with sperm DNA damage in Bos indicus bulls
Journal name Andrology   Check publisher's open access policy
ISSN 2047-2919
2047-2927
Publication date 2014-01-01
Year available 2014
Sub-type Article (original research)
DOI 10.1111/j.2047-2927.2014.00196.x
Open Access Status Not yet assessed
Volume 2
Issue 3
Start page 370
End page 378
Total pages 9
Place of publication Hoboken, NJ United States
Publisher Wiley-Blackwell Publishing Ltd
Language eng
Subject 1310 Endocrinology
2712 Endocrinology, Diabetes and Metabolism
2743 Reproductive Medicine
2748 Urology
Abstract The primary purpose of spermatozoa is to deliver the paternal DNA to the oocyte at fertilization. During the complex events of fertilization, if the spermatozoon penetrating the oocyte contains compromised or damaged sperm chromatin, the subsequent progression of embryogenesis and foetal development may be affected. Variation in sperm DNA damage and protamine content in ejaculated spermatozoa was reported in the cattle, with potential consequences to bull fertility. Protamines are sperm-specific nuclear proteins that are essential to packaging of the condensed paternal genome in spermatozoa. Sperm DNA damage is thought to be repaired during the process of protamination. This study investigates the potential correlation between sperm protamine content, sperm DNA damage and the subsequent relationships between sperm chromatin and commonly measured reproductive phenotypes. Bos indicus sperm samples (n = 133) were assessed by two flow cytometric methods: the sperm chromatin structure assay (SCSA) and an optimized sperm protamine deficiency assay (SPDA). To verify the SPDA assay for bovine sperm protamine content, samples collected from testis, caput and cauda epididymidis were analyzed. As expected, mature spermatozoa in the cauda epididymidis had higher protamine content when compared with sperm samples from testis and caput epididymidis (p < 0.01). The DNA fragmentation index (DFI), determined by SCSA, was positively correlated (r = 0.33 ± 0.08, p < 0.05) with the percentage of spermatozoa that showed low protamine content using SPDA. Also, DFI was negatively correlated (r = -0.21 ± 0.09, p < 0.05) with the percentage of spermatozoa with high protamine content. Larger scrotal circumference contributes to higher sperm protamine content and lower content of sperm DNA damage (p < 0.05). In conclusion, sperm protamine content and sperm DNA damage are closely associated. Protamine deficiency is likely to be one of the contributing factors to DNA instability and damage, which can affect bull fertility.
Keyword Bovine
DNA fragmentation
Sperm protamines
Spermatozoa
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID B.NBP.0787
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
Official 2015 Collection
School of Veterinary Science Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 8 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 9 times in Scopus Article | Citations
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Created: Tue, 20 May 2014, 11:46:59 EST by System User on behalf of Qld Alliance for Agriculture and Food Innovation