Transgenic flash mice for in vivo quantitative monitoring of canonical wnt signaling to track hair follicle cycle dynamics

Hodgson, Samantha S., Neufeld, Zoltan, Villani, Rehan M., Roy, Edwige and Khosrotehrani, Kiarash (2014) Transgenic flash mice for in vivo quantitative monitoring of canonical wnt signaling to track hair follicle cycle dynamics. Journal of Investigative Dermatology, 134 6: 1519-1526. doi:10.1038/jid.2014.92


Author Hodgson, Samantha S.
Neufeld, Zoltan
Villani, Rehan M.
Roy, Edwige
Khosrotehrani, Kiarash
Title Transgenic flash mice for in vivo quantitative monitoring of canonical wnt signaling to track hair follicle cycle dynamics
Formatted title
Transgenic flash mice for in vivo quantitative monitoring of canonical wnt signaling to track hair follicle cycle dynamics 
Journal name Journal of Investigative Dermatology   Check publisher's open access policy
ISSN 0022-202X
1523-1747
Publication date 2014-06-01
Year available 2014
Sub-type Article (original research)
DOI 10.1038/jid.2014.92
Open Access Status Not Open Access
Volume 134
Issue 6
Start page 1519
End page 1526
Total pages 8
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Language eng
Abstract Hair follicles (HFs) upon development enter a lifelong cycle of growth, regression, and resting. These phases have been extensively studied at the cellular and molecular levels for individual HFs. However, HFs group into domains with coordinated cycling strongly influenced by their environment. These macroscopic hair domains have been difficult to study and can be influenced by physiological or pathological conditions, such as pregnancy or skin wounds. To robustly address this issue, we generated a mouse model for quantitative monitoring of β-catenin activity reflecting HF cycle dynamics macroscopically by using live bioluminescence imaging. These mice allowed live tracking of HF cycles and development, and highlighted hair regenerative patterns known to occur through macro-environmental cues, including initiation events, propagating anagen and border stability, and allowed refinement of a mechanistic mathematical model that integrates epidermal cell population dynamics into an excitable reaction-diffusion model. HF cycling could be studied in situations of pregnancy, wound healing, hair plucking, as well as in response to cyclosporine or Wnt3a stimulation. In conclusion, we developed a model for analysis of HF cycling at the macroscopic level that will allow refined analysis of hair cycle kinetics as well as its propagation dynamics.
Keyword BLI
Bioluminescence
HFs
Hair follicles
WT
Wild type
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Mon, 19 May 2014, 22:22:11 EST by Rehan Villani on behalf of UQ Diamantina Institute