Differential effects of ultraviolet irradiation in neonatal versus adult mice are not explained by defective macrophage or neutrophil infiltration.

Rodero, Mathieu P., Hokando, Herlina Y., Villani, Rehan M., Walker, Graeme J. and Khosrotehrani, Kiarash (2014) Differential effects of ultraviolet irradiation in neonatal versus adult mice are not explained by defective macrophage or neutrophil infiltration.. Journal of Investigative Dermatology, 134 7: 1991-1997. doi:10.1038/jid.2014.78


Author Rodero, Mathieu P.
Hokando, Herlina Y.
Villani, Rehan M.
Walker, Graeme J.
Khosrotehrani, Kiarash
Title Differential effects of ultraviolet irradiation in neonatal versus adult mice are not explained by defective macrophage or neutrophil infiltration.
Journal name Journal of Investigative Dermatology   Check publisher's open access policy
ISSN 0022-202X
1523-1747
Publication date 2014-02-07
Sub-type Article (original research)
DOI 10.1038/jid.2014.78
Open Access Status Not Open Access
Volume 134
Issue 7
Start page 1991
End page 1997
Total pages 7
Place of publication London, United Kingdom
Publisher Nature Publishing
Language eng
Abstract Epidemiological studies suggest that ultraviolet B exposure (UVR) during childhood is the most important environmental risk factor for melanoma. In accordance, neonatal, but not adult, UVR exacerbates melanoma incidence in mouse models. The inability of neonates, as opposed to adults, to mount a proper neutrophil inflammatory response in the skin upon UVR exposure has been one of the driving hypotheses explaining this observation for the past decade. However, this aspect remains controversial. Here, we evaluated the UVR-induced inflammatory response in neonatal versus adult mice. In neonates, a significant neutrophil infiltration could be identified and quantified using three different antibodies by flow cytometry or immunohistochemistry. On day 1 after UVR, neutrophils were increased by 84-fold and on day 4 macrophages increased by 37-fold compared with nonexposed age-matched skin. When compared with adults, neonatal skin harbored a higher proportion of neutrophils in the myeloid compartment without significant differences in absolute counts. This response was reproduced with different kinetics in C57Bl/6 and FVB mice with a more rapid attenuation of neutrophil counts in the latter. Overall, our results suggest that the greatly increased sensitivity to melanomagenesis in neonates does not result from their incompetence in terms of myeloid inflammatory response to UVR.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Mon, 19 May 2014, 22:15:24 EST by Rehan Villani on behalf of UQ Diamantina Institute