Recombinant Escherichia coli strain produces a ZZ domain displaying biopolyester granules suitable for immunoglobulin G purification

Brockelbank, Jane A., Peters, Verena and Rehm, Bernd H. A. (2006) Recombinant Escherichia coli strain produces a ZZ domain displaying biopolyester granules suitable for immunoglobulin G purification. Applied and Environmental Microbiology, 72 11: 7394-7397. doi:10.1128/AEM.01014-06

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Author Brockelbank, Jane A.
Peters, Verena
Rehm, Bernd H. A.
Title Recombinant Escherichia coli strain produces a ZZ domain displaying biopolyester granules suitable for immunoglobulin G purification
Journal name Applied and Environmental Microbiology   Check publisher's open access policy
ISSN 0099-2240
1098-5336
Publication date 2006-11-01
Sub-type Article (original research)
DOI 10.1128/AEM.01014-06
Open Access Status File (Publisher version)
Volume 72
Issue 11
Start page 7394
End page 7397
Total pages 4
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Formatted abstract
The immunoglobulin G (IgG) binding ZZ domain of protein A from Staphylococcus aureus was fused to the N terminus of the polyhydroxyalkanoate (PHA) synthase from Cupriavidus necator. The fusion protein was confirmed by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry and mediated formation of ZZ domain-displaying PHA granules in recombinant Escherichia coll. The IgG binding capacity of isolated granules was assessed using enzyme-linked immunosorbent assay and could be enhanced by the overproduction of the ZZ-PHA synthase. ZZ-PIIA granules enabled efficient purification of IgG from human serum. 
Keyword Escherichia coli
Human serum
Purification of IgG
Staphylococcus aureus
Cupriavidus necator
Ionization-time-of-flight mass spectrometry
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Diamantina Institute - Open Access Collection
UQ Diamantina Institute Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 41 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 46 times in Scopus Article | Citations
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Created: Mon, 19 May 2014, 21:30:29 EST by Jane Mullaney on behalf of UQ Diamantina Institute