Protein engineering towards biotechnological production of bifunctional polyester beads

Atwood, Jane A. and Rehm, Bernd H. A. (2009) Protein engineering towards biotechnological production of bifunctional polyester beads. Biotechnology Letters, 31 1: 131-137. doi:10.1007/s10529-008-9836-9


Author Atwood, Jane A.
Rehm, Bernd H. A.
Title Protein engineering towards biotechnological production of bifunctional polyester beads
Journal name Biotechnology Letters   Check publisher's open access policy
ISSN 0141-5492
1573-6776
Publication date 2009-01-01
Sub-type Article (original research)
DOI 10.1007/s10529-008-9836-9
Open Access Status Not Open Access
Volume 31
Issue 1
Start page 131
End page 137
Total pages 7
Place of publication Dordrecht, The Netherlands
Publisher Springer Netherlands
Language eng
Subject 1305 Biotechnology
Abstract Microbial polyester inclusions have previously been demonstrated to be applicable as versatile beads outside the bacterial cell. Engineering of proteins selectively binding to the polyester inclusions was conceived to produce polyester beads simultaneously displaying two protein-based functions suitable for applications in, for example, fluorescence activated cell sorting (FACS). The polyester synthase and the phasin protein were fused to the green fluorescent protein (GFP) and the murine myelin oligodendrocyte glycoprotein (MOG), respectively, or GFP and MOG were fused to the N- and C-terminus, respectively, of only the phasin. In both cases, fusion proteins were found to be attached to isolated polyester inclusions while displaying both functionalities per bead. Functionalities at the bead surface were assessed by ELISA, FACS and fluorescence microscopy. The respective double fusion protein was identified by peptide fingerprinting using MALDI-TOF/MS.
Keyword Bio-beads
Biopolyester
Nanoparticle
Polyhydroxyalkanoate
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Diamantina Institute Publications
 
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Created: Mon, 19 May 2014, 21:29:22 EST by Jane Mullaney on behalf of UQ Diamantina Institute