Tuneable surface shear forces to physically displace nonspecific molecules in protein biomarker detection

Vaidyanathan, Ramanathan, Rauf, Sakandar, Shiddiky, Muhammad J. A. and Trau, Matt (2014) Tuneable surface shear forces to physically displace nonspecific molecules in protein biomarker detection. Biosensors and Bioelectronics, 61 184-191. doi:10.1016/j.bios.2014.03.061


Author Vaidyanathan, Ramanathan
Rauf, Sakandar
Shiddiky, Muhammad J. A.
Trau, Matt
Title Tuneable surface shear forces to physically displace nonspecific molecules in protein biomarker detection
Journal name Biosensors and Bioelectronics   Check publisher's open access policy
ISSN 0956-5663
1873-4235
Publication date 2014-11-15
Sub-type Article (original research)
DOI 10.1016/j.bios.2014.03.061
Open Access Status Not Open Access
Volume 61
Start page 184
End page 191
Total pages 8
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Abstract We report a simple method to remove nonspecifically adsorbed species from sensor surface and also improve the detection sensitivity of the sensor using tuneable alternating current (ac) electrohydrodynamics (ac-EHD) forces. These forces generated within few nanometers of an electrode surface (i.e., double layer) engender fluid flow within a serpentine channel containing a long array of the asymmetric electrode pairs, and can easily be tuned externally by changing the frequency and amplitude of the ac-EHD field. Under the optimized experimental conditions, we achieved a 3.5-fold reduction in nonspecific adsorption of non-target proteins with a 1000-fold enhancement in detection sensitivity of the device for the analysis of human epidermal growth factor receptor 2 (HER2) protein spiked in serum. This approach can be applicable in diverse fields including biosensors, cellular and molecular separation systems and biomedical applications to remove/reduce nonspecific adsorption of molecular and cellular species.
Keyword Electrohydrodynamics
Surface shear forces
Protein detection
Microfluidic devices
Nonspecific adsorption
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Fri, 16 May 2014, 21:17:42 EST by Ramanathan Pudhukode Vaidyanathan on behalf of Aust Institute for Bioengineering & Nanotechnology