In vivo delivery of bovine viral diahorrea virus, E2 protein using hollow mesoporous silica nanoparticles

Mahony, D., Cavallaro, A. S., Mody, K. T., Xiong, L., Mahony, T. J., Qiao, S. Z. and Mitter, Neena (2014) In vivo delivery of bovine viral diahorrea virus, E2 protein using hollow mesoporous silica nanoparticles. Nanoscale, 6 12: 6617-6626. doi:10.1039/C4NR01202J


Author Mahony, D.
Cavallaro, A. S.
Mody, K. T.
Xiong, L.
Mahony, T. J.
Qiao, S. Z.
Mitter, Neena
Title In vivo delivery of bovine viral diahorrea virus, E2 protein using hollow mesoporous silica nanoparticles
Formatted title
In vivo delivery of bovine viral diahorrea virus, E2 protein using hollow mesoporous silica nanoparticles
Journal name Nanoscale   Check publisher's open access policy
ISSN 2040-3364
2040-3372
Publication date 2014-04-11
Year available 2014
Sub-type Article (original research)
DOI 10.1039/C4NR01202J
Open Access Status Not Open Access
Volume 6
Issue 12
Start page 6617
End page 6626
Total pages 10
Place of publication Cambridge, United Kingdom
Publisher Royal Society of Chemistry
Language eng
Formatted abstract
Our work focuses on the application of mesoporous silica nanoparticles as a combined delivery vehicle and adjuvant for vaccine applications. Here we present results using the viral protein, E2, from bovine viral diarrhoea virus (BVDV). BVDV infection occurs in the target species of cattle and sheep herds worldwide and is therefore of economic importance. E2 is a major immunogenic determinant of BVDV and is an ideal candidate for the development of a subunit based nanovaccine using mesoporous silica nanoparticles. Hollow type mesoporous silica nanoparticles with surface amino functionalisation (termed HMSA) were characterised and assessed for adsorption and desorption of E2. A codon-optimised version of the E2 protein (termed Opti-E2) was produced in Escherichia coli. HMSA (120 nm) had an adsorption capacity of 80 µg Opti-E2 per mg HMSA and once bound E2 did not dissociate from the HMSA. Immunisation studies in mice with a 20 µg dose of E2 adsorbed to 250 mg HMSA was compared to immunisation with Opti-E2 (50 µg) together with the traditional adjuvant Quillaja saponaria Molina tree saponins (QuilA, 10 µg). The humoral responses with the Opti-E2/HMSA nanovaccine although slightly lower than those obtained for the Opti-E2 + QuilA group demonstrated that HMSA particles are an effective adjuvant that stimulated E2-specific antibody responses. Importantly the cell-mediated immune responses were consistently high in all mice immunised with Opti-E2/HMSA nanovaccine formulation. Therefore we have shown the Opti-E2/HMSA nanoformulation acts as an excellent adjuvant that gives both T-helper 1 and T-helper 2 mediated responses in a small animal model. This study has provided proof-of-concept towards the development of an E2 subunit nanoparticle based vaccine.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online 11 April 2014

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
Official 2015 Collection
 
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Citation counts: TR Web of Science Citation Count  Cited 17 times in Thomson Reuters Web of Science Article | Citations
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Created: Fri, 16 May 2014, 00:08:54 EST by Dr Neena Mitter on behalf of Qld Alliance for Agriculture and Food Innovation