Reduced peroxisomal citrate synthase activity increases substrate availability for polyhydroxyalkanoate biosynthesis in plant peroxisomes

Tilbrook, Kimberley, Poirier, Yves, Gebbie, Leigh, Schenk, Peer M., McQualter, Richard B. and Brumbley, Stevens M. (2014) Reduced peroxisomal citrate synthase activity increases substrate availability for polyhydroxyalkanoate biosynthesis in plant peroxisomes. Plant Biotechnology Journal, 12 8: 1044-1052. doi:10.1111/pbi.12211


Author Tilbrook, Kimberley
Poirier, Yves
Gebbie, Leigh
Schenk, Peer M.
McQualter, Richard B.
Brumbley, Stevens M.
Title Reduced peroxisomal citrate synthase activity increases substrate availability for polyhydroxyalkanoate biosynthesis in plant peroxisomes
Journal name Plant Biotechnology Journal   Check publisher's open access policy
ISSN 1467-7644
1467-7652
Publication date 2014-06-19
Year available 2014
Sub-type Article (original research)
DOI 10.1111/pbi.12211
Open Access Status DOI
Volume 12
Issue 8
Start page 1044
End page 1052
Total pages 9
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell
Language eng
Formatted abstract
Polyhydroxyalkanoates (PHAs) are bacterial carbon storage polymers used as renewable, biodegradable plastics. PHA production in plants may be a way to reduce industrial PHA production costs. We recently demonstrated a promising level of peroxisomal PHA production in the high biomass crop species sugarcane. However, further production strategies are needed to boost PHA accumulation closer to commercial targets. Through exogenous fatty acid feeding of Arabidopsis thaliana plants that contain peroxisome-targeted PhaA, PhaB and PhaC enzymes from Cupriavidus necator, we show here that the availability of substrates derived from the β-oxidation cycle limits peroxisomal polyhydroxybutyrate (PHB) biosynthesis. Knockdown of peroxisomal citrate synthase activity using artificial microRNA increased PHB production levels approximately threefold. This work demonstrates that reduction of peroxisomal citrate synthase activity may be a valid metabolic engineering strategy for increasing PHA production in other plant species. 
Keyword Polyhydroxyalkanoate
Citrate synthase
β-oxidation
Peroxisome
Plant metabolic engineering
Arabidopsis
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 31003A-138339
Institutional Status UQ

 
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Created: Thu, 15 May 2014, 20:12:07 EST by Dr Richard Mcqualter on behalf of Aust Institute for Bioengineering & Nanotechnology