The fate of chromosomes and alleles in an allohexaploid Brassica population

Mason, Annaliese S., Nelson, Matthew N., Takahira, Junko, Cowling, Wallace A., Moreira Alves, Gustavo, Chaudhuri, Arkaprava, Chen, Ning, Ragu, Mohana E., Dalton-Morgan, Jessica, Coriton, Olivier, Huteau, Virginie, Eber, Frédérique, Chèvre, Anne-Marie and Batley, Jacqueline (2014) The fate of chromosomes and alleles in an allohexaploid Brassica population. Genetics, 197 1: 273-283. doi:10.1534/genetics.113.159574

Author Mason, Annaliese S.
Nelson, Matthew N.
Takahira, Junko
Cowling, Wallace A.
Moreira Alves, Gustavo
Chaudhuri, Arkaprava
Chen, Ning
Ragu, Mohana E.
Dalton-Morgan, Jessica
Coriton, Olivier
Huteau, Virginie
Eber, Frédérique
Chèvre, Anne-Marie
Batley, Jacqueline
Title The fate of chromosomes and alleles in an allohexaploid Brassica population
Formatted title
The fate of chromosomes and alleles in an allohexaploid Brassica population
Journal name Genetics   Check publisher's open access policy
ISSN 1943-2631
Publication date 2014-05-01
Sub-type Article (original research)
DOI 10.1534/genetics.113.159574
Open Access Status Not Open Access
Volume 197
Issue 1
Start page 273
End page 283
Total pages 11
Place of publication Bethesda, MD, United States
Publisher Genetics Society of America
Language eng
Formatted abstract
Production of allohexaploid Brassica (2n = AABBCC) is a promising goal for plant breeders due to the potential for hybrid heterosis and useful allelic contributions from all three of the Brassica genomes present in the cultivated diploid (2n = AA, 2n = BB, 2n = CC) and allotetraploid (2n = AABB, 2n = AACC, and 2n = BBCC) crop species (canola, cabbages, mustards). We used highthroughput SNP molecular marker assays, flow cytometry, and fluorescent in situ hybridization (FISH) to characterize a population of putative allohexaploids derived from self-pollination of a hybrid from the novel cross (B. napus 3 B. carinata) 3 B. juncea to investigate whether fertile, stable allohexaploid Brassica can be produced. Allelic segregation in the A and C genomes generally followed Mendelian expectations for an F2 population, with minimal nonhomologous chromosome pairing. However, we detected no strong selection for complete 2n = AABBCC chromosome complements, with weak correlations between DNA content and fertility (r2 = 0.11) and no correlation between missing chromosomes or chromosome segments and fertility. Investigation of next-generation progeny resulting from one highly fertile F2 plant using FISH revealed general maintenance of high chromosome numbers but severe distortions in karyotype, as evidenced by recombinant chromosomes and putative loss/duplication of A- and C-genome chromosome pairs. Our results show promise for the development of meiotically stable allohexaploid lines, but highlight the necessity of selection for 2n = AABBCC karyotypes.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Centre for Integrative Legume Research Publications
School of Agriculture and Food Sciences
Official 2015 Collection
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Citation counts: TR Web of Science Citation Count  Cited 5 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 11 times in Scopus Article | Citations
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Created: Thu, 15 May 2014, 20:02:52 EST by Annaliese Mason on behalf of School of Agriculture and Food Sciences