Pleiotropic mutations are subject to strong stabilizing selection

McGuigan, Katrina, Collet, Julie M., Allen, Scott L., Chenoweth, Stephen F. and Blows, Mark W. (2014) Pleiotropic mutations are subject to strong stabilizing selection. Genetics, 197 3: 1051-1062. doi:10.1534/genetics.114.165720


Author McGuigan, Katrina
Collet, Julie M.
Allen, Scott L.
Chenoweth, Stephen F.
Blows, Mark W.
Title Pleiotropic mutations are subject to strong stabilizing selection
Journal name Genetics   Check publisher's open access policy
ISSN 0016-6731
1943-2631
Publication date 2014-05-02
Sub-type Article (original research)
DOI 10.1534/genetics.114.165720
Open Access Status Not Open Access
Volume 197
Issue 3
Start page 1051
End page 1062
Total pages 12
Place of publication Bethesda, MD, United States
Publisher Genetics Society of America
Language eng
Formatted abstract
The assumption that pleiotropic mutations are more deleterious than mutations with more restricted phenotypic effects is an important premise in models of evolution. However, empirical evidence supporting this assumption is limited. Here, we estimated the strength of stabilizing selection on mutations affecting gene expression in male Drosophila serrata. We estimated the mutational variance (VM) and the standing genetic variance (VG) from two well-matched panels of inbred lines; a panel of mutation accumulation (MA) lines derived from a single inbred ancestral line, and a panel of inbred lines derived from an outbred population. For 855 gene expression traits, we estimated the strength of stabilizing selection as s = VM/VG. Selection was observed to be relatively strong, with 17% of traits having s >0.02, a magnitude typically associated with life history traits. Randomly assigning expression traits to five-trait sets, we used factor analytic mixed modeling in the MA dataset to identify covarying traits that shared pleiotropic mutations. By assigning traits to the same trait sets in the outbred line dataset, we then estimated s for the combination of traits affected by pleiotropic mutation. For these pleiotropic combinations, the median s was three times greater than s acting on the individual component traits, and 46% of the pleiotropic trait combinations had s >0.02. Although our analytical approach was biased toward detecting mutations with relatively large effects, likely overestimating the average strength of selection, our results provide widespread support for the prediction that stronger selection can act against mutations with pleiotropic effects.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Biological Sciences Publications
 
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Created: Wed, 14 May 2014, 22:56:26 EST by Julie Collet on behalf of School of Biological Sciences