Association mapping of resistance to Puccinia hordei in Australian barley breeding germplasm

Ziems, L. A., Hickey, L. T., Hunt, C. H., Mace, E. S., Platz, G. J., Franckowiak, J. D. and Jordan, D. R. (2014) Association mapping of resistance to Puccinia hordei in Australian barley breeding germplasm. Theoretical and Applied Genetics, 127 5: 1199-1212. doi:10.1007/s00122-014-2291-1


Author Ziems, L. A.
Hickey, L. T.
Hunt, C. H.
Mace, E. S.
Platz, G. J.
Franckowiak, J. D.
Jordan, D. R.
Title Association mapping of resistance to Puccinia hordei in Australian barley breeding germplasm
Formatted title
Association mapping of resistance to Puccinia hordei in Australian barley breeding germplasm
Journal name Theoretical and Applied Genetics   Check publisher's open access policy
ISSN 0040-5752
1432-2242
Publication date 2014-05-01
Sub-type Article (original research)
DOI 10.1007/s00122-014-2291-1
Volume 127
Issue 5
Start page 1199
End page 1212
Total pages 14
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Formatted abstract
Key message: “To find stable resistance using association mapping tools, QTL with major and minor effects on leaf rust reactions were identified in barley breeding lines by assessing seedlings and adult plants.”

Three hundred and sixty (360) elite barley (Hordeum vulgare L.) breeding lines from the Northern Region Barley Breeding Program in Australia were genotyped with 3,244 polymorphic diversity arrays technology markers and the results used to map quantitative trait loci (QTL) conferring a reaction to leaf rust (Puccinia hordei Otth). The F3:5 (Stage 2) lines were derived or sourced from different geographic origins or hubs of international barley breeding ventures representing two breeding cycles (2009 and 2011 trials) and were evaluated across eight environments for infection type at both seedling and adult plant stages. Association mapping was performed using mean scores for disease reaction, accounting for family effects using the eigenvalues from a matrix of genotype correlations. In this study, 15 QTL were detected; 5 QTL co-located with catalogued leaf rust resistance genes (Rph1, Rph3/19, Rph8/14/15, Rph20, Rph21), 6 QTL aligned with previously reported genomic regions and 4 QTL (3 on chromosome 1H and 1 on 7H) were novel. The adult plant resistance gene Rph20 was identified across the majority of environments and pathotypes. The QTL detected in this study offer opportunities for breeding for more durable resistance to leaf rust through pyramiding multiple genomic regions via marker-assisted selection.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Communicated by X. Qi.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
Official 2015 Collection
 
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Created: Wed, 14 May 2014, 00:41:41 EST by Lee Hickey on behalf of Qld Alliance for Agriculture and Food Innovation