Role of capsule and O antigen in the virulence of uropathogenic Escherichia coli

Sarkar, Sohinee, Ulett, Glen C., Totsika, Makrina, Phan, Minh-Duy and Schembri, Mark A. (2014) Role of capsule and O antigen in the virulence of uropathogenic Escherichia coli. PLoS One, 9 4: . doi:10.1371/journal.pone.0094786


Author Sarkar, Sohinee
Ulett, Glen C.
Totsika, Makrina
Phan, Minh-Duy
Schembri, Mark A.
Title Role of capsule and O antigen in the virulence of uropathogenic Escherichia coli
Formatted title
Role of capsule and O antigen in the virulence of uropathogenic Escherichia coli
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2014-04-10
Year available 2014
Sub-type Article (original research)
DOI 10.1371/journal.pone.0094786
Open Access Status DOI
Volume 9
Issue 4
Total pages 11
Place of publication San Francisco, United States
Publisher Public Library of Science
Language eng
Formatted abstract
Urinary tract infection (UTI) is one of the most common bacterial infections in humans, with uropathogenic Escherichia coli (UPEC) the leading causative organism. UPEC has a number of virulence factors that enable it to overcome host defenses within the urinary tract and establish infection. The O antigen and the capsular polysaccharide are two such factors that provide a survival advantage to UPEC. Here we describe the application of the rpsL counter selection system to construct capsule (kpsD) and O antigen (waaL) mutants and complemented derivatives of three reference UPEC strains: CFT073 (O6:K2:H1), RS218 (O18:K1:H7) and 1177 (O1:K1:H7). We observed that while the O1, O6 and O18 antigens were required for survival in human serum, the role of the capsule was less clear and linked to O antigen type. In contrast, both the K1 and K2 capsular antigens provided a survival advantage to UPEC in whole blood. In the mouse urinary tract, mutation of the O6 antigen significantly attenuated CFT073 bladder colonization. Overall, this study contrasts the role of capsule and O antigen in three common UPEC serotypes using defined mutant and complemented strains. The combined mutagenesis complementation strategy can be applied to study other virulence factors with complex functions both in vitro and in vivo.
Keyword Multidisciplinary Sciences
Science & Technology - Other Topics
MULTIDISCIPLINARY SCIENCES
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID APP1012076
FT110101048
DE130101169
Institutional Status UQ
Additional Notes Article # e94786

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Chemistry and Molecular Biosciences
 
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Created: Wed, 07 May 2014, 18:31:20 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences