Differential sRNA regulation in leaves and roots of sugarcane under water depletion

Thiebaut, Flavia, Grativol, Clı´cia, Tanurdzic, Milos, Carnavale-Bottino, Mariana, Vieira, Tauan, Motta, Mariana Romeiro, Rojas, Cristian, Vincentini, Renato, Chabregas, Sabrina Moutinho, Hemerly, Adriana Silva, Martienssen, Robert A. and Ferreira, Paulo Cavalcanti Gomes (2014) Differential sRNA regulation in leaves and roots of sugarcane under water depletion. PLoS One, 9 4: e93822.1-e93822.17. doi:10.1371/journal.pone.0093822

Author Thiebaut, Flavia
Grativol, Clı´cia
Tanurdzic, Milos
Carnavale-Bottino, Mariana
Vieira, Tauan
Motta, Mariana Romeiro
Rojas, Cristian
Vincentini, Renato
Chabregas, Sabrina Moutinho
Hemerly, Adriana Silva
Martienssen, Robert A.
Ferreira, Paulo Cavalcanti Gomes
Title Differential sRNA regulation in leaves and roots of sugarcane under water depletion
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2014-04-02
Year available 2014
Sub-type Article (original research)
DOI 10.1371/journal.pone.0093822
Open Access Status DOI
Volume 9
Issue 4
Start page e93822.1
End page e93822.17
Total pages 17
Place of publication San Francisco, CA United States
Publisher Public Library of Science
Language eng
Subject 1100 Agricultural and Biological Sciences
1300 Biochemistry, Genetics and Molecular Biology
2700 Medicine
Abstract Plants have developed multiple regulatory mechanisms to respond and adapt to stress. Drought stress is one of the major constraints to agricultural productivity worldwide and recent reports have highlighted the importance of plant sRNA in the response and adaptation to water availability. In order to increase our understanding of the roles of sRNA in response to water depletion, cultivars of sugarcane were submitted to treatment of ceasing drip irrigation for 24 hours. Deep sequencing analysis was carried out to identify the sRNA regulated in leaves and roots of sugarcane cultivars with different drought sensitivities. The pool of sRNA selected allowed the analysis of different sRNA classes (miRNA and siRNA). Twentyeight and 36 families of conserved miRNA were identified in leaf and root libraries, respectively. Dynamic regulation of miRNA was observed and the expression profiles of eight miRNA were verified in leaf samples from three biological replicates by stem-loop qRT-PCR assay using the cultivars: SP90-1638 - sensitive cultivar- and; SP83-2847 and SP83-5073 - tolerant cultivars. Altered miRNA regulation was correlated with changes in mRNA levels of specific targets. Two leaf libraries from individual sugarcane cultivars with contrasting drought-tolerance properties were also analyzed. An enrichment of 22-nt sRNA species was observed in leaf libraries. 22-nt miRNA triggered siRNA production by cleavage of their targets in response to water depletion. A number of genes of the sRNA biogenesis pathway were down-regulated in tolerant genotypes and up-regulated in sensitive in response to water depletion treatment. Our analysis contributes to increase the knowledge on the roles of sRNA in sugarcane submitted to water depletion.
Keyword Multidisciplinary Sciences
Science & Technology - Other Topics
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
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