An improved high-performance liquid chromatographic method for the simultaneous measurement of halofantrine and desbutylhalofantrine in human serum

Keeratithakul D., Teja-Isavadharm P., Shanks G.D., Webster H.K. and Edstein M.D. (1991) An improved high-performance liquid chromatographic method for the simultaneous measurement of halofantrine and desbutylhalofantrine in human serum. Therapeutic Drug Monitoring, 13 1: 64-68. doi:10.1097/00007691-199101000-00009


Author Keeratithakul D.
Teja-Isavadharm P.
Shanks G.D.
Webster H.K.
Edstein M.D.
Title An improved high-performance liquid chromatographic method for the simultaneous measurement of halofantrine and desbutylhalofantrine in human serum
Journal name Therapeutic Drug Monitoring   Check publisher's open access policy
ISSN 0163-4356
Publication date 1991-01-01
Year available 1991
Sub-type Article (original research)
DOI 10.1097/00007691-199101000-00009
Open Access Status Not yet assessed
Volume 13
Issue 1
Start page 64
End page 68
Total pages 5
Place of publication PHILADELPHIA
Publisher LIPPINCOTT-RAVEN PUBL
Language eng
Subject 1300 Biochemistry, Genetics and Molecular Biology
1303 Specialist Studies in Education
2307 Health, Toxicology and Mutagenesis
2736 Pharmacology (medical)
2739 Public Health, Environmental and Occupational Health
3004 Pharmacology
3005 Toxicology
Abstract A rapid, accurate, and sensitive high-performance liquid chromatographic (HPLC) method, with ultraviolet detection, for simultaneous measurement of halofantrine (HAL) and desbutylhalofantrine (BHAL) in human serum is described. Sample preparation involved protein precipitation, followed by a solid-phase clean-up and a liquid-liquid extraction. Chromatographic separation was carried out on an Ultrasphere C8 column (25 cm x 4.6 mm I.D., 5 mu-m particle size) using a mobile phase of acetonitrile: water, 75:25, (vol/vol), with 0.2% (w/vol) sodium dodecyl sulfate and 0.2% (vol/vol) glacial acetic acid. The serum sample volume used was 0.5 ml, with concentrations normalized to 1 ml. Retention times for BHAL, HAL, and the internal standard were 5.5, 8.3, and 11.5 min, respectively, with a total run time of 13.5 min. The average extraction recovery for HAL was 85.6% and 100.5% for BHAL. Inter- and intra-assay precisions of HAL and BHAL were less-than-or-equal-to 7.5%, with an accuracy of less-than-or-equal-to 10.8% over three concentrations (0.02, 0.5, 2.0-mu-g/ml). Detection limits of HAL and BHAL were 5 and 3 ng/ml, respectively. The new HPLC method resulted in cleaner chromatograms and a 1.7-fold faster run time than previous HPLC methods. Application of the method with clinical specimens was demonstrated.
Keyword Desbutylhalofantrine
Halofantrine
High-performance liquid chromatography
Malaria
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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