High-resolution molecular karyotyping uncovers pairing between ancestrally related Brassica chromosomes

Mason, Annaliese S., Batley, Jacqueline, Bayer, Philipp Emanuel, Hayward, Alice, Cowling, Wallace A. and Nelson, Matthew N. (2014) High-resolution molecular karyotyping uncovers pairing between ancestrally related Brassica chromosomes. New Phytologist, 202 3: 964-974. doi:10.1111/nph.12706


Author Mason, Annaliese S.
Batley, Jacqueline
Bayer, Philipp Emanuel
Hayward, Alice
Cowling, Wallace A.
Nelson, Matthew N.
Title High-resolution molecular karyotyping uncovers pairing between ancestrally related Brassica chromosomes
Journal name New Phytologist   Check publisher's open access policy
ISSN 1469-8137
Publication date 2014-01-01
Sub-type Article (original research)
DOI 10.1111/nph.12706
Open Access Status Not Open Access
Volume 202
Issue 3
Start page 964
End page 974
Total pages 11
Place of publication Oxford, United Kingdom
Publisher Blackwell Publishing Ltd
Language eng
Subject 1110 Nursing
1314 Physiology
Abstract How do chromosomal regions with differing degrees of homology and homeology interact at meiosis? We provide a novel analytical method based on simple genetics principles which can help to answer this important question. This method interrogates high-throughput molecular marker data in order to infer chromosome behavior at meiosis in interspecific hybrids. We validated this method using high-resolution molecular marker karyotyping in two experimental Brassica populations derived from interspecific crosses among B. juncea, B. napus and B. carinata, using a single nucleotide polymorphism chip. This method of analysis successfully identified meiotic interactions between chromosomes sharing different degrees of similarity: full-length homologs; full-length homeologs; large sections of primary homeologs; and small sections of secondary homeologs. This analytical method can be applied to any allopolyploid species or fertile interspecific hybrid in order to detect meiotic associations. This genetic information can then be used to identify which genomic regions share functional homeology (i.e., retain enough similarity to allow pairing and segregation at meiosis). When applied to interspecific hybrids for which reference genome sequences are available, the question of how differing degrees of homology and homeology affect meiotic interactions may finally be resolved.
Keyword Brassica
Meiosis
Molecular karyotyping
Polyploidy
Recombination
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Centre for Integrative Legume Research Publications
School of Agriculture and Food Sciences
Official 2015 Collection
 
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Citation counts: TR Web of Science Citation Count  Cited 14 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 15 times in Scopus Article | Citations
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Created: Thu, 01 May 2014, 19:48:44 EST by Annaliese Mason on behalf of School of Agriculture and Food Sciences