Chromosome-Specific Microsatellite Sets for Fluorescence-Based, Semiautomated Genome Mapping

Reed, PW, Davies, JL, Copeman, JB, Bennett, ST, Palmer, SM, Pritchard, LE, Gough, Scl, Kawaguchi, Y, Cordell, HJ, Balfour, KM, Jenkins, SC, Powell, EE, Vignal, A and Todd, JA (1994) Chromosome-Specific Microsatellite Sets for Fluorescence-Based, Semiautomated Genome Mapping. Nature Genetics, 7 3: 390-395. doi:10.1038/ng0794-390


Author Reed, PW
Davies, JL
Copeman, JB
Bennett, ST
Palmer, SM
Pritchard, LE
Gough, Scl
Kawaguchi, Y
Cordell, HJ
Balfour, KM
Jenkins, SC
Powell, EE
Vignal, A
Todd, JA
Title Chromosome-Specific Microsatellite Sets for Fluorescence-Based, Semiautomated Genome Mapping
Journal name Nature Genetics   Check publisher's open access policy
ISSN 1061-4036
Publication date 1994-07-01
Year available 1994
Sub-type Article (original research)
DOI 10.1038/ng0794-390
Open Access Status Not yet assessed
Volume 7
Issue 3
Start page 390
End page 395
Total pages 6
Place of publication NEW YORK
Publisher NATURE PUBLISHING CO
Language eng
Abstract To facilitate large-scale genetic mapping of the human genome, we have developed chromosome-specific sets of microsatellite marker loci suitable for use with a fluorescence-based automated DNA fragment analyser. We present 254 dinucleotide repeat marker loci (80% from the Genethon genetic linkage map) arranged into 39 sets, covering all 22 autosomes and the X chromosome. The average distance between adjacent markers is 13 centiMorgans, and less than 4% of the genome lies more than 20 cM from the nearest marker. Each set of microsatellites consists of up to nine marker loci, with allele size ranges that do not overlap. We selected marker loci on the basis of their reliability in the polymerase chain reaction, polymorphism content, map position and the accuracy with which alleles can be scored automatically by the Genotyper(TM) program.
Keyword Markers
Loci
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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