An immunomics approach to schistosome antigen discovery: antibody signatures of naturally resistant and chronically infected individuals from endemic areas

Gaze, Soraya, Driguez, Patrick, Pearson, Mark S., Mendes, Tiago, Doolan, Denise L., Trieu, Angela, McManus, Donald P., Gobert, Geoffrey N., Periago, Maria Victoria, Correa Oliveira, Rodrigo, Cardoso, Fernanda C., Oliveira, Guilherme, Nakajima, Rie, Jasinskas, Al, Hung, Chris, Liang, Li, Pablo, Jozelyn, Bethony, Jeffrey M., Felgner, Philip L. and Loukas, Alex (2014) An immunomics approach to schistosome antigen discovery: antibody signatures of naturally resistant and chronically infected individuals from endemic areas. PLoS Pathogens, 10 3: e1004033.1-e1004033.16. doi:10.1371/journal.ppat.1004033

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Author Gaze, Soraya
Driguez, Patrick
Pearson, Mark S.
Mendes, Tiago
Doolan, Denise L.
Trieu, Angela
McManus, Donald P.
Gobert, Geoffrey N.
Periago, Maria Victoria
Correa Oliveira, Rodrigo
Cardoso, Fernanda C.
Oliveira, Guilherme
Nakajima, Rie
Jasinskas, Al
Hung, Chris
Liang, Li
Pablo, Jozelyn
Bethony, Jeffrey M.
Felgner, Philip L.
Loukas, Alex
Title An immunomics approach to schistosome antigen discovery: antibody signatures of naturally resistant and chronically infected individuals from endemic areas
Journal name PLoS Pathogens   Check publisher's open access policy
ISSN 1553-7374
1553-7366
Publication date 2014-03-27
Sub-type Article (original research)
DOI 10.1371/journal.ppat.1004033
Open Access Status DOI
Volume 10
Issue 3
Start page e1004033.1
End page e1004033.16
Total pages 16
Editor Stephen John Davies
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Language eng
Subject 2404 Microbiology
2405 Parasitology
2406 Virology
2403 Immunology
1311 Genetics
1312 Molecular Biology
Formatted abstract
Schistosomiasis is a neglected tropical disease that is responsible for almost 300,000 deaths annually. Mass drug administration (MDA) is used worldwide for the control of schistosomiasis, but chemotherapy fails to prevent reinfection with schistosomes, so MDA alone is not sufficient to eliminate the disease, and a prophylactic vaccine is required. Herein, we take advantage of recent advances in systems biology and longitudinal studies in schistosomiasis endemic areas in Brazil to pilot an immunomics approach to the discovery of schistosomiasis vaccine antigens. We selected mostly surface-derived proteins, produced them using an in vitro rapid translation system and then printed them to generate the first protein microarray for a multi-cellular pathogen. Using well-established Brazilian cohorts of putatively resistant (PR) and chronically infected (CI) individuals stratified by the intensity of their S. mansoni infection, we probed arrays for IgG subclass and IgE responses to these antigens to detect antibody signatures that were reflective of protective vs. non-protective immune responses. Moreover, probing for IgE responses allowed us to identify antigens that might induce potentially deleterious hypersensitivity responses if used as subunit vaccines in endemic populations. Using multi-dimensional cluster analysis we showed that PR individuals mounted a distinct and robust IgG1 response to a small set of newly discovered and well-characterized surface (tegument) antigens in contrast to CI individuals who mounted strong IgE and IgG4 responses to many antigens. Herein, we show the utility of a vaccinomics approach that profiles antibody responses of resistant individuals in a high-throughput multiplex approach for the identification of several potentially protective and safe schistosomiasis vaccine antigens.
Keyword Schistosome
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Medicine Publications
Institute for Molecular Bioscience - Publications
 
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