Rhinovirus exacerbates house-dust-mite induced lung disease in adult mice

Phan, Jennifer A., Kicic, Anthony, Berry, Luke J., Fernandes, Lynette B., Zosky, Graeme R., Sly, Peter D. and Larcombe, Alexander N. (2014) Rhinovirus exacerbates house-dust-mite induced lung disease in adult mice. Plos One, 9 3: e92163.1-e92163.10. doi:10.1371/journal.pone.0092163

Author Phan, Jennifer A.
Kicic, Anthony
Berry, Luke J.
Fernandes, Lynette B.
Zosky, Graeme R.
Sly, Peter D.
Larcombe, Alexander N.
Title Rhinovirus exacerbates house-dust-mite induced lung disease in adult mice
Journal name Plos One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2014-03-01
Year available 2014
Sub-type Article (original research)
DOI 10.1371/journal.pone.0092163
Open Access Status DOI
Volume 9
Issue 3
Start page e92163.1
End page e92163.10
Total pages 10
Place of publication San Francisco United States
Publisher Public Library of Science
Language eng
Formatted abstract
Human rhinovirus is a key viral trigger for asthma exacerbations. To date, murine studies investigating rhinovirus-induced exacerbation of allergic airways disease have employed systemic sensitisation/intranasal challenge with ovalbumin. In this study, we combined human-rhinovirus infection with a clinically relevant mouse model of aero-allergen exposure using house-dust-mite in an attempt to more accurately understand the links between human-rhinovirus infection and exacerbations of asthma. Adult BALB/c mice were intranasally exposed to low-dose house-dust-mite (or vehicle) daily for 10 days. On day 9, mice were inoculated with human-rhinovirus-1B (or UV-inactivated human-rhinovirus-1B). Forty-eight hours after inoculation, we assessed bronchoalveolar cellular inflammation, levels of relevant cytokines/serum antibodies, lung function and responsiveness/sensitivity to methacholine. House-dust-mite exposure did not result in a classical TH2-driven response, but was more representative of noneosinophilic asthma. However, there were significant effects of house-dust-mite exposure on most of the parameters measured including increased cellular inflammation (primarily macrophages and neutrophils), increased total IgE and house-dust-mite-specific IgG1 and increased responsiveness/sensitivity to methacholine. There were limited effects of human-rhinovirus-1B infection alone, and the combination of the two insults resulted in additive increases in neutrophil levels and lung parenchymal responses to methacholine (tissue elastance). We conclude that acute rhinovirus infection exacerbates house-dust-mite-induced lung disease in adult mice. The similarity of our results using the naturally occurring allergen house-dust-mite, to previous studies using ovalbumin, suggests that the exacerbation of allergic airways disease by rhinovirus infection could act via multiple or conserved mechanisms.
Keyword Allergic airway inflammation
Subjects in-vivo
Asthmatic subjects
Chronic bronchitis
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Queensland Children's Medical Research Institute Publications
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Citation counts: TR Web of Science Citation Count  Cited 12 times in Thomson Reuters Web of Science Article | Citations
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