Limited dengue virus replication in field-collected Aedes aegypti mosquitoes infected with Wolbachia

Frentiu, Francesca D., Zakir, Tasnim, Walker, Thomas, Popovici, Jean, Pyke, Alyssa T., van den Hurk, Andrew, McGraw, Elizabeth A. and O'Neill, Scott L. (2014) Limited dengue virus replication in field-collected Aedes aegypti mosquitoes infected with Wolbachia. PLoS Neglected Tropical Diseases, 8 2: e2688.1-e2688.10. doi:10.1371/journal.pntd.0002688


Author Frentiu, Francesca D.
Zakir, Tasnim
Walker, Thomas
Popovici, Jean
Pyke, Alyssa T.
van den Hurk, Andrew
McGraw, Elizabeth A.
O'Neill, Scott L.
Title Limited dengue virus replication in field-collected Aedes aegypti mosquitoes infected with Wolbachia
Formatted title
Limited dengue virus replication in field-collected Aedes aegypti mosquitoes infected with Wolbachia
Journal name PLoS Neglected Tropical Diseases   Check publisher's open access policy
ISSN 1935-2735
1935-2727
Publication date 2014-02-01
Sub-type Article (original research)
DOI 10.1371/journal.pntd.0002688
Open Access Status DOI
Volume 8
Issue 2
Start page e2688.1
End page e2688.10
Total pages 10
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Language eng
Formatted abstract
Introduction: Dengue is one of the most widespread mosquito-borne diseases in the world. The causative agent, dengue
virus (DENV), is primarily transmitted by the mosquito Aedes aegypti, a species that has proved difficult to control using
conventional methods. The discovery that A. aegypti transinfected with the wMel strain of Wolbachia showed limited DENV
replication led to trial field releases of these mosquitoes in Cairns, Australia as a biocontrol strategy for the virus.

Methodology/Principal Findings: Field collected wMel mosquitoes that were challenged with three DENV serotypes
displayed limited rates of body infection, viral replication and dissemination to the head compared to uninfected controls.
Rates of dengue infection, replication and dissemination in field wMel mosquitoes were similar to those observed in the
original transinfected wMel line that had been maintained in the laboratory. We found that wMel was distributed in similar
body tissues in field mosquitoes as in laboratory ones, but, at seven days following blood-feeding, wMel densities increased
to a greater extent in field mosquitoes.

Conclusions/Significance: Our results indicate that virus-blocking is likely to persist in Wolbachia-infected mosquitoes after
their release and establishment in wild populations, suggesting that Wolbachia biocontrol may be a successful strategy for
reducing dengue transmission in the field.
Keyword Drosophila-Simulans
Density
Transmission
Strain
Populations
Chikungunya
Inhibition
Adaptation
Albopictus
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Institute for Molecular Bioscience - Publications
 
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