Ultraviolet light-induced G2 phase cell cycle checkpoint blocks cdc25-dependent progression into mitosis

Gabrielli, BG, Clark, JM, McCormack, AK and Ellem, KAO (1997) Ultraviolet light-induced G2 phase cell cycle checkpoint blocks cdc25-dependent progression into mitosis. Oncogene, 15 7: 749-758. doi:10.1038/sj.onc.1201254


Author Gabrielli, BG
Clark, JM
McCormack, AK
Ellem, KAO
Title Ultraviolet light-induced G2 phase cell cycle checkpoint blocks cdc25-dependent progression into mitosis
Journal name Oncogene   Check publisher's open access policy
ISSN 0950-9232
Publication date 1997-08-01
Year available 1997
Sub-type Article (original research)
DOI 10.1038/sj.onc.1201254
Open Access Status
Volume 15
Issue 7
Start page 749
End page 758
Total pages 10
Place of publication BASINGSTOKE
Publisher STOCKTON PRESS
Language eng
Abstract In response to low doses of ultraviolet (U.V.) radiation, cells undergo a G2 delay. In this study we have shown that the G2 delay results in the accumulation of inactive forms of cyclin B1/cdc2 and both the G2 and mitotic complexes of cyclin A/cdk. This appears to be through a block in the cdc25-dependent activation of these complexes. The expression and localisation of cyclin A and cyclin B1/cdk complexes are similar in U.V.-induced G2 delay and normal early G2 phase cells, Cdc25B and cdc25C also accumulate to normal G2 levels in U.V. irradiated cells, but the mitotic phosphorylation associated with increased activity of both cdc25B and cdc25C is absent. The cdc25B accumulates in the nucleus of U.V. irradiated cells and in normal G2 phase cells. Thus the block in cyclin B/cdc2 activation is in part due to the physical separation of cyclin B/cdc2, localised in the cytoplasm, from the cdc25B and cdc25C phosphatases localised in the nucleus, The data positions the U.V.-induced G2 checkpoint at either the S/G2 transition or early G2 phase, prior to the activation of cyclin A/cdk2.
Keyword cdc25
cell cycle checkpoint
Uv
Maturation-Promoting Factor
Cdk2 Protein-Kinase
Ionizing-Radiation
Human Fibroblasts
Hela-Cells
Dependent Kinases
Nitrogen-Mustard
Dna-Replication
B Irradiation
G1 Arrest
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 51 times in Thomson Reuters Web of Science Article | Citations
Google Scholar Search Google Scholar
Created: Sat, 19 Apr 2014, 04:22:15 EST by System User