Requirement for Cdk2 in Cytostatic Factor Mediated Metaphase-Ii Arrest

Gabrielli, BG, Roy, LM and Maller, JL (1993) Requirement for Cdk2 in Cytostatic Factor Mediated Metaphase-Ii Arrest. Science, 259 5102: 1766-1769. doi:10.1126/science.8456304


Author Gabrielli, BG
Roy, LM
Maller, JL
Title Requirement for Cdk2 in Cytostatic Factor Mediated Metaphase-Ii Arrest
Journal name Science   Check publisher's open access policy
ISSN 0036-8075
Publication date 1993-03-01
Year available 1993
Sub-type Article (original research)
DOI 10.1126/science.8456304
Open Access Status
Volume 259
Issue 5102
Start page 1766
End page 1769
Total pages 4
Place of publication WASHINGTON
Publisher AMER ASSOC ADVANCEMENT SCIENCE
Language eng
Abstract The unfertilized eggs of vertebrates are arrested in metaphase of meiosis II because of the activity of cytostatic factor (CSF). Xenopus CSF is thought to contain the product of the Mos proto-oncogene, but other proteins synthesized during meiosis II are also required for arrest induced by CSF. In Xenopus oocytes, ablation of synthesis of cyclin-dependent kinase 2 (Cdk2) during meiosis resulted in absence of the metaphase II block, even though the Mos(xe) protein kinase was fully active at metaphase. Introduction of purified Cdk2 restored metaphase II arrest, and increasing the amount of Cdk2 during meiosis I (when Mos(xe) is present) led to metaphase arrest at meiosis I. These data indicate that metaphase arrest is a result of cooperation between a proto-oncogene kinase and a cyclin-dependent kinase and illustrate the interaction of a cell growth regulator with a cell cycle control element.
Keyword Maturation-Promoting Factor
Proto-Oncogene Product
Meiotic Maturation
Xenopus Eggs
Protooncogene Product
Mosxe Protooncogene
Cytoplasmic Factor
Cyclin-a
M-Phase
Oocytes
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID F32 CA0981
GM26743
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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