A cdc2-related kinase oscillates in the cell cycle independently of cyclins G2/M and cdc2

Gabrielli, Brian G., Roy, Linda M., Gautier, Jean, Philippe, Michel and Maller, James L. (1992) A cdc2-related kinase oscillates in the cell cycle independently of cyclins G2/M and cdc2. Journal of Biological Chemistry, 267 3: 1969-1975.

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Author Gabrielli, Brian G.
Roy, Linda M.
Gautier, Jean
Philippe, Michel
Maller, James L.
Title A cdc2-related kinase oscillates in the cell cycle independently of cyclins G2/M and cdc2
Formatted title
A cdc2-related kinase oscillates in the cell cycle independently of cyclins G2/M and cdc2
Journal name Journal of Biological Chemistry   Check publisher's open access policy
ISSN 0021-9258
1083-351X
Publication date 1992-01-25
Year available 1992
Sub-type Article (original research)
Open Access Status File (Publisher version)
Volume 267
Issue 3
Start page 1969
End page 1975
Total pages 7
Place of publication Bethesda, MD, United States
Publisher American Society for Biochemistry and Molecular Biology
Language eng
Formatted abstract
The Eg1 gene in Xenopus laevis is related in sequence to the cdc2+ gene. We show here that the Eg1 gene product (cdk2) possesses histone H1 protein kinase activity and binds to PSTAIR antibodies as well as to Sepharose beads linked to the 13-kDa product of the suc 1 gene (p13suc1). Eg1 protein kinase is active only in an Mr ≈ 200,000 complex with other proteins but is not associated with any of the three known Xenopus mitotic cyclins or with any newly synthesized protein in egg extracts that exhibit cell cycle oscillations in vitro. The protein kinase activity of Eg1 oscillates in the mitotic cell cycle, being high in M-phase and low in interphase. Hyperactivation of cdc2 kinase by the addition of cyclin A has no effect on the activity or oscillatory behavior of Eg1. Inhibition of cdc2 kinase activation by emetine or RNase treatment of oscillating extracts does not inhibit the activation of Eg1 but does block deactivation normally seen during exit from mitosis. These results indicate that Eg1 is regulated by a cell cycle clock independently of cyclin and cdc2 kinase.
Keyword Biochemistry & Molecular Biology
Biochemistry & Molecular Biology
BIOCHEMISTRY & MOLECULAR BIOLOGY
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID F32-CA08914
GM26743
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Mater Research Institute-UQ (MRI-UQ)
 
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