General in vitro caspase assay procedures

Boucher, Dave, Duclos, Catherine and Denault, Jean-Bernard (2014). General in vitro caspase assay procedures. In Peter V. Bozhkov and Guy Salvesen (Ed.), Caspases, Paracaspases, and Metacaspases: Methods and Protocols (pp. 3-39) New York, NY, United States: Humana Press. doi:10.1007/978-1-4939-0357-3_1


Author Boucher, Dave
Duclos, Catherine
Denault, Jean-Bernard
Title of chapter General in vitro caspase assay procedures
Title of book Caspases, Paracaspases, and Metacaspases: Methods and Protocols
Place of Publication New York, NY, United States
Publisher Humana Press
Publication Year 2014
Sub-type Research book chapter (original research)
DOI 10.1007/978-1-4939-0357-3_1
Series Methods in Molecular Biology
ISBN 9781493903566
9781493903573
ISSN 1064-3745
1940-6029
Editor Peter V. Bozhkov
Guy Salvesen
Volume number 1133
Chapter number 1
Start page 3
End page 39
Total pages 37
Total chapters 16
Language eng
Abstract/Summary One of the most valuable tools that have been developed for the study of apoptosis is the availability of recombinant active caspases. The determination of caspase substrate preference, the design of sensitive substrates and potent inhibitors, the resolution of caspase structures, the elucidation of their activation mechanisms, and the identification of their substrates were made possible by the availability of sufficient amounts of enzymatically pure caspases. The current chapter describes at length the expression, purification, and basic enzymatic characterization of apoptotic caspases.
Keyword Active-site titration
Caspase
Enzymatic assays
Purification
Q-Index Code BX
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Tue, 15 Apr 2014, 11:34:56 EST by System User on behalf of Institute for Molecular Bioscience