Elevated metal concentrations in the CF airway correlate with cellular injury and disease severity

Smith, Daniel J., Anderson, Gregory J., Bell, Scott C. and Reid, David W. (2014) Elevated metal concentrations in the CF airway correlate with cellular injury and disease severity. Journal of Cystic Fibrosis, 13 3: 289-295. doi:10.1016/j.jcf.2013.12.001


Author Smith, Daniel J.
Anderson, Gregory J.
Bell, Scott C.
Reid, David W.
Title Elevated metal concentrations in the CF airway correlate with cellular injury and disease severity
Journal name Journal of Cystic Fibrosis   Check publisher's open access policy
ISSN 1873-5010
1569-1993
Publication date 2014-05-01
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.jcf.2013.12.001
Open Access Status
Volume 13
Issue 3
Start page 289
End page 295
Total pages 7
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Subject 2735 Pediatrics, Perinatology, and Child Health
2740 Pulmonary and Respiratory Medicine
Abstract Background: Bio-active trace metals have been identified in respiratory tract secretions of subjects with lung disease and may potentially influence bacterial virulence, inflammation and disease severity. We measured a diverse range of metal ions in sputum samples from subjects with CF and non-CF bronchiectasis (NCFB) compared to healthy controls and examined their relationship to airway inflammation, disease severity and the presence of bacterial pathogens. Methods: We studied 45 subjects with CF, 8 with NCFB and 8 healthy controls. Metal concentrations were measured in sputum supernatant by inductively-coupled plasma mass spectrometry and correlated with sputum inflammatory cell counts, lactate dehydrogenase (LDH) and interleukin (IL)-8 concentrations, atmospheric particulate matter, lung function, clinical status and participant demographics. Results: Sputum from subjects with CF and NCFB contained increased concentrations of magnesium, calcium, iron and zinc. Metal ion concentrations correlated positively with LDH levels. The concentrations of magnesium, iron and zinc positively correlated with IL-8. A sub-group of CF subjects with severe lung disease demonstrated increased sputum molybdenum concentrations. Conclusion: Elevated concentrations of sputum metal ions appear to be associated with cell/tissue necrosis and inflammation in subjects with CF and NCFB. Sputum molybdenum concentrations may be a biomarker of severe CF airway disease.
Formatted abstract
Background
Bio-active trace metals have been identified in respiratory tract secretions of subjects with lung disease and may potentially influence bacterial virulence, inflammation and disease severity. We measured a diverse range of metal ions in sputum samples from subjects with CF and non-CF bronchiectasis (NCFB) compared to healthy controls and examined their relationship to airway inflammation, disease severity and the presence of bacterial pathogens.

Methods
We studied 45 subjects with CF, 8 with NCFB and 8 healthy controls. Metal concentrations were measured in sputum supernatant by inductively-coupled plasma mass spectrometry and correlated with sputum inflammatory cell counts, lactate dehydrogenase (LDH) and interleukin (IL)-8 concentrations, atmospheric particulate matter, lung function, clinical status and participant demographics.

Results
Sputum from subjects with CF and NCFB contained increased concentrations of magnesium, calcium, iron and zinc. Metal ion concentrations correlated positively with LDH levels. The concentrations of magnesium, iron and zinc positively correlated with IL-8. A sub-group of CF subjects with severe lung disease demonstrated increased sputum molybdenum concentrations.

Conclusion
Elevated concentrations of sputum metal ions appear to be associated with cell/tissue necrosis and inflammation in subjects with CF and NCFB. Sputum molybdenum concentrations may be a biomarker of severe CF airway disease.
Keyword Bio-metals
Cellular injury
Cystic Fibrosis
Inflammation
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
Queensland Children's Medical Research Institute Publications
School of Medicine Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 12 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 13 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 15 Apr 2014, 11:18:25 EST by System User on behalf of School of Medicine