Bioleaching in brackish waters-effect of chloride ions on the acidophile population and proteomes of model species

Zammit, Carla M., Mangold, Stefanie, Rao Jonna, Venkateswara, Mutch, Lesley A., Watling, Helen R., Dopson, Mark and Watkin, Elizabeth L. J. (2012) Bioleaching in brackish waters-effect of chloride ions on the acidophile population and proteomes of model species. Applied Microbiology and Biotechnology, 93 1: 319-329. doi:10.1007/s00253-011-3731-3


Author Zammit, Carla M.
Mangold, Stefanie
Rao Jonna, Venkateswara
Mutch, Lesley A.
Watling, Helen R.
Dopson, Mark
Watkin, Elizabeth L. J.
Title Bioleaching in brackish waters-effect of chloride ions on the acidophile population and proteomes of model species
Journal name Applied Microbiology and Biotechnology   Check publisher's open access policy
ISSN 0175-7598
1432-0614
Publication date 2012-01-01
Sub-type Article (original research)
DOI 10.1007/s00253-011-3731-3
Volume 93
Issue 1
Start page 319
End page 329
Total pages 11
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Abstract High concentrations of chloride ions inhibit the growth of acidophilic microorganisms used in biomining, a problem particularly relevant to Western Australian and Chilean biomining operations. Despite this, little is known about the mechanisms acidophiles adopt in order to tolerate high chloride ion concentrations. This study aimed to investigate the impact of increasing concentrations of chloride ions on the population dynamics of a mixed culture during pyrite bioleaching and apply proteomics to elucidate how two species from this mixed culture alter their proteomes under chloride stress. A mixture consisting of well-known biomining microorganisms and an enrichment culture obtained from an acidic saline drain were tested for their ability to bioleach pyrite in the presence of 0, 3.5, 7, and 20 g∈L -1 NaCl. Microorganisms from the enrichment culture were found to out-compete the known biomining microorganisms, independent of the chloride ion concentration. The proteomes of the Gram-positive acidophile Acidimicrobium ferrooxidans and the Gram-negative acidophile Acidithiobacillus caldus grown in the presence or absence of chloride ions were investigated. Analysis of differential expression showed that acidophilic microorganisms adopted several changes in their proteomes in the presence of chloride ions, suggesting the following strategies to combat the NaCl stress: adaptation of the cell membrane, the accumulation of amino acids possibly as a form of osmoprotectant, and the expression of a YceI family protein involved in acid and osmotic-related stress.
Keyword Biomining
Brackish
Chloride
Membrane
Proteomics
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Earth Sciences Publications
 
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