Phylogenetic analysis of mitochondrial genome sequences indicates that the cattle tick, Rhipicephalus (Boophilus) microplus, contains a cryptic species

Burger, Thomas D., Shao, Renfu and Barker, Stephen C. (2014) Phylogenetic analysis of mitochondrial genome sequences indicates that the cattle tick, Rhipicephalus (Boophilus) microplus, contains a cryptic species. Molecular Phylogenetics and Evolution, 76 1: 241-253. doi:10.1016/j.ympev.2014.03.017


Author Burger, Thomas D.
Shao, Renfu
Barker, Stephen C.
Title Phylogenetic analysis of mitochondrial genome sequences indicates that the cattle tick, Rhipicephalus (Boophilus) microplus, contains a cryptic species
Formatted title
Phylogenetic analysis of mitochondrial genome sequences indicates that the cattle tick, Rhipicephalus (Boophilus) microplus, contains a cryptic species
Journal name Molecular Phylogenetics and Evolution   Check publisher's open access policy
ISSN 1055-7903
1095-9513
Publication date 2014-07-01
Sub-type Article (original research)
DOI 10.1016/j.ympev.2014.03.017
Volume 76
Issue 1
Start page 241
End page 253
Total pages 13
Place of publication Maryland Heights, MO, United States
Publisher Academic Press
Language eng
Formatted abstract
Highlights
• We sequenced the entire or partial mitochondrial genomes of five species of Rhipicephlus (Boophilus).
• We also sequenced the entire mitochondrial genomes of three specimens of R. microplus from Brazil, Cambodia, and China.
• Our analyses suggest that R. microplus from China and India are a cryptic species.
• Aditionally, we assessed the phylogenetic resolution within the R. microplus complex of: cox1, ITS2, 12S and 16S rRNA.
• Of these four common markers, cox1 and 16S rRNA have the best phylogenetic resolution within the R. microplus complex.

Cattle ticks of the subgenus Rhipicephalus (Boophilus) are major agricultural pests worldwide, causing billions of dollars in losses annually. Rhipicephalus (Boophilus) annulatus and R. microplus are the most well-known and widespread species, and a third species, R. australis, was recently reinstated for ‘R. microplus’ from Australia and parts of Southeast Asia. We use mitochondrial genome sequences to address the phylogenetic relationships among the species of the subgenus Boophilus. We sequenced the complete or partial mitochondrial genomes of R. annulatus, R. australis, R. kohlsi, R. geigyi, and of three geographically disparate specimens of R. microplus from Brazil, Cambodia and China. Phylogenetic analyses of mitochondrial genomes, as well as cox1 and 16S rRNA sequences, reveals a species complex of R. annulatus, R. australis, and two clades of R. microplus, which we call the R. microplus complex. We show that cattle ticks morphologically identified as R. microplus from Southern China and Northern India (R. microplus clade B) are more closely related to R. annulatus than other specimens of R. microplus s.s. from Asia, South America and Africa (R. microplus clade A). Our analysis suggests that ticks reported as R. microplus from Southern China and Northern India are a cryptic species. This highlights the need for further molecular, morphological and crossbreeding studies of the R. microplus complex, with emphasis on specimens from China and India. We found that cox1 and, to a lesser extent, 16S rRNA were far more successful in resolving the phylogenetic relationships within the R. microplus complex than 12S rRNA or the nuclear marker ITS2. We suggest that future molecular studies of the R. microplus complex should focus on cox1, supplemented by 16S rRNA, and develop nuclear markers alternative to ITS2 to complement the mitochondrial data.
Keyword Cattle ticks
Rhipicephalus (Boophilus) microplus
Cryptic species
Mitochondrial genomes
Phylogenetics
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Chemistry and Molecular Biosciences
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 32 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 33 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 09 Apr 2014, 21:08:04 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences