Immune cell infiltration in malignant middle cerebral artery infarction: comparison with transient cerebral ischemia

Chu, Hannah X., Kim, Hyun A., Lee, Seyoung, Moore, Jeffrey P., Chan, Christopher T., Vinh, Antony, Gelderblom, Mathias, Arumugam, Thiruma V., Broughton, Brad R. S., Drummond, Grant R. and Sobey, Christopher G. (2014) Immune cell infiltration in malignant middle cerebral artery infarction: comparison with transient cerebral ischemia. Journal of Cerebral Blood Flow and Metabolism, 34 3: 450-459. doi:10.1038/jcbfm.2013.217


Author Chu, Hannah X.
Kim, Hyun A.
Lee, Seyoung
Moore, Jeffrey P.
Chan, Christopher T.
Vinh, Antony
Gelderblom, Mathias
Arumugam, Thiruma V.
Broughton, Brad R. S.
Drummond, Grant R.
Sobey, Christopher G.
Title Immune cell infiltration in malignant middle cerebral artery infarction: comparison with transient cerebral ischemia
Journal name Journal of Cerebral Blood Flow and Metabolism   Check publisher's open access policy
ISSN 0271-678X
1559-7016
Publication date 2014-03-01
Year available 2013
Sub-type Article (original research)
DOI 10.1038/jcbfm.2013.217
Open Access Status DOI
Volume 34
Issue 3
Start page 450
End page 459
Total pages 10
Place of publication New York, United States
Publisher Nature
Language eng
Formatted abstract
We tested whether significant leukocyte infiltration occurs in a mouse model of permanent cerebral ischemia. C57BL6/J male mice underwent either permanent (3 or 24 hours) or transient (1 or 2 hours+22- to 23-hour reperfusion) middle cerebral artery occlusion (MCAO). Using flow cytometry, we observed ∼15,000 leukocytes (CD45+high cells) in the ischemic hemisphere as early as 3 hours after permanent MCAO (pMCAO), comprising ∼40% lymphoid cells and ∼60% myeloid cells. Neutrophils were the predominant cell type entering the brain, and were increased to ∼5,000 as early as 3 hours after pMCAO. Several cell types (monocytes, macrophages, B lymphocytes, CD8+ T lymphocytes, and natural killer cells) were also increased at 3 hours to levels sustained for 24 hours, whereas others (CD4+ T cells, natural killer T cells, and dendritic cells) were unchanged at 3 hours, but were increased by 24 hours after pMCAO. Immunohistochemical analysis revealed that leukocytes typically had entered and widely dispersed throughout the parenchyma of the infarct within 3 hours. Moreover, compared with pMCAO, there were ∼50% fewer infiltrating leukocytes at 24 hours after transient MCAO (tMCAO), independent of infarct size. Microglial cell numbers were bilaterally increased in both models. These findings indicate that a profound infiltration of inflammatory cells occurs in the brain early after focal ischemia, especially without reperfusion.
Keyword Immune cell infiltration
Inflammation
Leukocytes
Malignant middle cerebral artery infarction
Middle cerebral artery occlusion
Stroke
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Biomedical Sciences Publications
 
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