Thermoresponsive worms for expansion and release of human embryonic stem cells

Chen, Xiaoli, Prowse, Andrew B. J., Jia, Zhongfan, Tellier, Helena, Munro,Trent P., Gray, Peter P. and Monteiro, Michael J. (2014) Thermoresponsive worms for expansion and release of human embryonic stem cells. Biomacromolecules, 15 3: 844-855. doi:10.1021/bm401702h

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Author Chen, Xiaoli
Prowse, Andrew B. J.
Jia, Zhongfan
Tellier, Helena
Munro,Trent P.
Gray, Peter P.
Monteiro, Michael J.
Title Thermoresponsive worms for expansion and release of human embryonic stem cells
Journal name Biomacromolecules   Check publisher's open access policy
ISSN 1525-7797
Publication date 2014-03-10
Year available 2014
Sub-type Article (original research)
DOI 10.1021/bm401702h
Open Access Status File (Author Post-print)
Volume 15
Issue 3
Start page 844
End page 855
Total pages 12
Place of publication Washington, DC United States
Publisher American Chemical Society
Language eng
Subject 1502 Banking, Finance and Investment
2505 Materials Chemistry
2507 Polymers and Plastics
2502 Biomaterials
Abstract The development of robust suspension cultures of human embryonic stem cells (hESCs) without the use of cell membrane disrupting enzymes or inhibitors is critical for future clinical applications in regenerative medicine. We have achieved this by using long, flexible, and thermoresponsive polymer worms decorated with a recombinant vitronectin subdomain that bridge hESCs, aiding in hESC's natural ability to form embryoid bodies (EBs) and satisfying their inherent requirement for cell-cell and cell-extracellular matrix contact. When the EBs reached an optimal upper size where cytokine and nutrient penetration becomes limiting, these long and flexible polymer worms facilitated EB breakdown via a temperature shift from 37 to 25 C. The thermoresponsive nature of the worms enabled a cyclical dissociation and propagation of the cells. Repeating the process for three cycles (over eighteen days) provided a >30-fold expansion in cell number while maintaining pluripotency, thereby providing a simple, nondestructive process for the 3D expansion of hESC.
Keyword Biochemistry & Molecular Biology
Chemistry, Organic
Polymer Science
Biochemistry & Molecular Biology
Polymer Science
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
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Citation counts: TR Web of Science Citation Count  Cited 13 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 14 times in Scopus Article | Citations
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