Biological performance of a polycaprolactone-based scaffold plus recombinant human morphogenetic protein-2 (rhBMP-2) in an ovine thoracic interbody fusion model

Yong, M.R.N.O., Saifzadeh, S., Askin, G.N., Labrom, R.D., Hutmacher, D.W. and Adam, C.J. (2014) Biological performance of a polycaprolactone-based scaffold plus recombinant human morphogenetic protein-2 (rhBMP-2) in an ovine thoracic interbody fusion model. European Spine Journal, 23 3: 650-657. doi:10.1007/s00586-013-3085-x

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Author Yong, M.R.N.O.
Saifzadeh, S.
Askin, G.N.
Labrom, R.D.
Hutmacher, D.W.
Adam, C.J.
Title Biological performance of a polycaprolactone-based scaffold plus recombinant human morphogenetic protein-2 (rhBMP-2) in an ovine thoracic interbody fusion model
Journal name European Spine Journal   Check publisher's open access policy
ISSN 1432-0932
0940-6719
Publication date 2014-01-01
Sub-type Article (original research)
DOI 10.1007/s00586-013-3085-x
Open Access Status DOI
Volume 23
Issue 3
Start page 650
End page 657
Total pages 8
Place of publication Heidelberg, Germany
Publisher Springer Verlag
Language eng
Subject 2746 Surgery
2732 Orthopedics and Sports Medicine
Abstract Purpose: We develop a sheep thoracic spine interbody fusion model to study the suitability of polycaprolactone-based scaffold and recombinant human bone morphogenetic protein-2 (rhBMP-2) as a bone graft substitute within the thoracic spine. The surgical approach is a mini-open thoracotomy with relevance to minimally invasive deformity correction surgery for adolescent idiopathic scoliosis. To date there are no studies examining the use of this biodegradable implant in combination with biologics in a sheep thoracic spine model. Methods: In the present study, six sheep underwent a 3-level (T6/7, T8/9 and T10/11) discectomy with randomly allocated implantation of a different graft substitute at each of the three levels: (a) calcium phosphate (CaP) coated polycaprolactone-based scaffold plus 0.54 μg rhBMP-2 (b) CaP-coated PCL-based scaffold alone or (c) autograft (mulched rib head). Fusion was assessed at 6 months post-surgery. Results: Computed Tomographic scanning demonstrated higher fusion grades in the rhBMP-2 plus PCL-based scaffold group in comparison with either PCL-based scaffold alone or autograft. These results were supported by histological evaluations of the respective groups. Biomechanical testing revealed significantly higher stiffness for the rhBMP-2 plus PCL-based scaffold group in all loading directions in comparison with the other two groups. Conclusion: The results of this study demonstrate that rhBMP-2 plus PCL-based scaffold is a viable bone graft substitute, providing an optimal environment for thoracic interbody spinal fusion in a large animal model.
Keyword Animal model
Bone tissue engineering
Growth factors
Polycaprolactone
Spinal fusion
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Medicine Publications
 
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Created: Mon, 07 Apr 2014, 23:46:41 EST by Matthew Lamb on behalf of School of Medicine