Genome-wide analysis of ruminant Staphylococcus aureus reveals diversification of the core genome

Zakour, Nouri L. Ben, Sturdevant, Daniel E., Even, Sergine, Guinane, Catriona M., Barbey, Corinne, Alves, Priscila D., Cochet, Marie-Francoise, Gautier, Michel, Otto, Michael, Fitzgerald, J. Ross and Le Loir, Yves (2008) Genome-wide analysis of ruminant Staphylococcus aureus reveals diversification of the core genome. Journal of Bacteriology, 190 19: 6302-6317. doi:10.1128/JB.01984-07

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Author Zakour, Nouri L. Ben
Sturdevant, Daniel E.
Even, Sergine
Guinane, Catriona M.
Barbey, Corinne
Alves, Priscila D.
Cochet, Marie-Francoise
Gautier, Michel
Otto, Michael
Fitzgerald, J. Ross
Le Loir, Yves
Title Genome-wide analysis of ruminant Staphylococcus aureus reveals diversification of the core genome
Journal name Journal of Bacteriology   Check publisher's open access policy
ISSN 0021-9193
Publication date 2008-01-01
Year available 2008
Sub-type Article (original research)
DOI 10.1128/JB.01984-07
Open Access Status File (Publisher version)
Volume 190
Issue 19
Start page 6302
End page 6317
Total pages 16
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Abstract Staphylococcus aureus causes disease in humans and a wide array of animals. Of note, S. aureus mastitis of ruminants, including cows, sheep, and goats, results in major economic losses worldwide. Extensive variation in genome content exists among S. aureus pathogenic clones. However, the genomic variation among S. aureus strains infecting different animal species has not been well examined. To investigate variation in the genome content of human and ruminant S. aureus, we carried out whole-genome PCR scanning (WGPS), comparative genomic hybridizations (CGH), and the directed DNA sequence analysis of strains of human, bovine, ovine, and caprine origin. Extensive variation in genome content was discovered, including host- and ruminant-specific genetic loci. Ovine and caprine strains were genetically allied, whereas bovine strains were heterogeneous in gene content. As expected, mobile genetic elements such as pathogenicity islands and bacteriophages contributed to the variation in genome content between strains. However, differences specific for ruminant strains were restricted to regions of the conserved core genome, which contained allelic variation in genes encoding proteins of known and unknown function. Many of these proteins are predicted to be exported and could play a role in host-pathogen interactions. The genomic regions of difference identified by the whole-genome approaches adopted in the current study represent excellent targets for studies of the molecular basis of S. aureus host adaptation. Copyright
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Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
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Citation counts: TR Web of Science Citation Count  Cited 39 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 03 Apr 2014, 01:37:26 EST by Nouri Ben Zakour on behalf of Examinations