Tandem repeat sequence analysis of staphylococcal protein A (spa) gene in methicillin-resistant Staphylococcus pseudintermedius

Moodley, Arshnee, Stegger, Marc, Ben Zakour, Nouri L., Fitzgerald, J. Ross and Guardabassi, Luca (2009) Tandem repeat sequence analysis of staphylococcal protein A (spa) gene in methicillin-resistant Staphylococcus pseudintermedius. Veterinary Microbiology, 135 3-4: 320-326. doi:10.1016/j.vetmic.2008.09.070


Author Moodley, Arshnee
Stegger, Marc
Ben Zakour, Nouri L.
Fitzgerald, J. Ross
Guardabassi, Luca
Title Tandem repeat sequence analysis of staphylococcal protein A (spa) gene in methicillin-resistant Staphylococcus pseudintermedius
Journal name Veterinary Microbiology   Check publisher's open access policy
ISSN 0378-1135
1873-2542
Publication date 2009-03-30
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.vetmic.2008.09.070
Volume 135
Issue 3-4
Start page 320
End page 326
Total pages 7
Place of publication Amsterdam, The Netherlands
Publisher Elsevier BV
Language eng
Subject 2404 Microbiology
3400 Veterinary
Abstract A putative staphylococcal protein A (spa) gene was discovered in the genome of Staphylococcus pseudintermedius and used for developing a species-specific spa typing protocol. Thirty-one clinical methicillin-resistant S. pseudintermedius (MRSP) isolates from dogs and cats in four countries were characterized by spa typing, pulsed-field gel electrophoresis (PFGE) and staphylococcal cassette chromosome (SCCmec) typing. The results indicated the occurrence of two MRSP clones that acquired distinct SCCmec elements in Europe (t02, PFGE type A, SCCmec type III,) and California (t06, PFGE type B, SCCmec type V). Sequence analysis of mecA revealed the occurrence of four alleles (mecA1 to mecA4), which correlated with the geographical origin of the isolates and enabled discrimination of two distinct subtypes within the European clone. The newly developed spa typing method appeared to be a promising tool for easy and rapid typing of MRSP, either alone or in combination with SCCmec and mecA typing for fine-structure epidemiological analysis.
Keyword Antibiotic resistance
Epidemiology
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 57 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 59 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Thu, 03 Apr 2014, 01:28:22 EST by Nouri Ben Zakour on behalf of School of Chemistry & Molecular Biosciences