Staphylococcus aureus virulence expression is impaired by Lactococcus lactis in mixed cultures

Even, Sergine, Charlier, Cathy, Nouaille, Sebastien, Ben Zakour, Nouri L., Cretenet, Marina, Cousin, Fabien J., Gautier, Michel, Cocaign-Bousquet, Muriel, Loubiere, Pascal and Le Loir, Yves (2009) Staphylococcus aureus virulence expression is impaired by Lactococcus lactis in mixed cultures. Applied and Environmental Microbiology, 75 13: 4459-4472. doi:10.1128/AEM.02388-08

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Author Even, Sergine
Charlier, Cathy
Nouaille, Sebastien
Ben Zakour, Nouri L.
Cretenet, Marina
Cousin, Fabien J.
Gautier, Michel
Cocaign-Bousquet, Muriel
Loubiere, Pascal
Le Loir, Yves
Title Staphylococcus aureus virulence expression is impaired by Lactococcus lactis in mixed cultures
Journal name Applied and Environmental Microbiology   Check publisher's open access policy
ISSN 0099-2240
1098-5336
Publication date 2009-01-01
Year available 2009
Sub-type Article (original research)
DOI 10.1128/AEM.02388-08
Open Access Status File (Publisher version)
Volume 75
Issue 13
Start page 4459
End page 4472
Total pages 14
Place of publication Washington, DC United State
Publisher American Society for Microbiology
Language eng
Subject 2402 Applied Microbiology and Biotechnology
1106 Human Movement and Sports Science
1305 Biotechnology
2303 Ecology
Abstract Staphylococcus aureus is responsible for numerous food poisonings due to the production of enterotoxins by strains contaminating foodstuffs, especially dairy products. Several parameters, including interaction with antagonistic flora such as Lactococcus lactis, a lactic acid bacterium widely used in the dairy industry, can modulate S. aureus proliferation and virulence expression. We developed a dedicated S. aureus microarray to investigate the effect of L. lactis on staphylococcal gene expression in mixed cultures. This microarray was used to establish the transcriptomic profile of S. aureus in mixed cultures with L. lactis in a chemically defined medium held at a constant pH (6.6). Under these conditions, L. lactis hardly affected S. aureus growth. The expression of most genes involved in the cellular machinery, carbohydrate and nitrogen metabolism, and stress responses was only slightly modulated: a short time lag in mixed compared to pure cultures was observed. Interestingly, the induction of several virulence factors and regulators, including the agr locus, sarA, and some enterotoxins, was strongly affected. This work clearly underlines the complexity of L. lactis antagonistic potential for S. aureus and yields promising leads for investigations into nonantibiotic biocontrol of this major pathogen. Copyright
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Supplemental material (read only) - http://aem.asm.org/content/75/13/4459/suppl/DC1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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Created: Thu, 03 Apr 2014, 01:27:23 EST by Nouri Ben Zakour on behalf of Examinations