Freeze-drying of ovalbumin loaded mesoporous silica nanoparticle vaccine formulation increases antigen stability under ambient conditions

Mody, Karishma T., Mahony, Donna, Cavallaro, Antonino S., Stahr, Frances, Qiao, Shi Zhang, Mahony, Timothy J. and Mitter, Neena (2014) Freeze-drying of ovalbumin loaded mesoporous silica nanoparticle vaccine formulation increases antigen stability under ambient conditions. International Journal of Pharmaceutics, 465 1-2: 325-332. doi:10.1016/j.ijpharm.2014.01.037


Author Mody, Karishma T.
Mahony, Donna
Cavallaro, Antonino S.
Stahr, Frances
Qiao, Shi Zhang
Mahony, Timothy J.
Mitter, Neena
Title Freeze-drying of ovalbumin loaded mesoporous silica nanoparticle vaccine formulation increases antigen stability under ambient conditions
Journal name International Journal of Pharmaceutics   Check publisher's open access policy
ISSN 0378-5173
1873-3476
Publication date 2014-05-01
Year available 2014
Sub-type Article (original research)
DOI 10.1016/j.ijpharm.2014.01.037
Open Access Status Not yet assessed
Volume 465
Issue 1-2
Start page 325
End page 332
Total pages 8
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Abstract Amino functionalised mesoporous silica nanoparticles (AM-41) have been identified as a promising vaccine delivery material. The capacity of AM-41 to stabilise vaccine components at ambient temperature (23-27 °C) was determined by adsorbing the model antigen ovalbumin (OVA) to AM-41 particles (OVA-41). The OVA-41 was successfully freeze-dried using the excipients 5% trehalose and 1% PEG8000. The immunological activity of OVA and the nanoparticle structure were maintained following two months storage at ambient temperature. The results of immunisation studies in mice with reconstituted OVA-41 demonstrated the induction of humoral and cell-meditated immune responses. The capacity of AM-41 particles to facilitate ambient storage of vaccine components without the loss of immunological potency will underpin the further development of this promising vaccine delivery platform.
Keyword Excipients
Freeze-drying
Mesoporous silica nanoparticles
Protein
Stability
Vaccine delivery
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Wed, 02 Apr 2014, 21:29:15 EST by Anthony Yeates on behalf of Qld Alliance for Agriculture and Food Innovation