Hydrophilic interaction liquid chromatography-tandem mass spectrometric determination of creatinine in human urine

Hewavitharana, Amitha K., Darch, Katherine M. and Shaw, P. Nicholas (2014) Hydrophilic interaction liquid chromatography-tandem mass spectrometric determination of creatinine in human urine. Analytical Letters, 47 4: 689-696. doi:10.1080/00032719.2013.848363


Author Hewavitharana, Amitha K.
Darch, Katherine M.
Shaw, P. Nicholas
Title Hydrophilic interaction liquid chromatography-tandem mass spectrometric determination of creatinine in human urine
Journal name Analytical Letters   Check publisher's open access policy
ISSN 0003-2719
1532-236X
Publication date 2014-01-01
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1080/00032719.2013.848363
Volume 47
Issue 4
Start page 689
End page 696
Total pages 8
Place of publication Philadelphia, PA, United States
Publisher Taylor & Francis
Language eng
Formatted abstract
The ability to measure creatinine with good accuracy is essential as its urinary concentration is used to quantify other analytes in urinalysis. In this study, an assay for the determination of creatinine in human urine by hydrophilic interaction liquid chromatography and electrospray ionization tandem mass spectrometry was developed and validated. Hydrophilic interaction chromatography provided retention of creatinine well away from the early eluting impurities that can reduce sensitivity by suppression of the mass spectrometry signal. Ion suppression effects were further eliminated by using a co-eluting, stable-isotope labeled creatinine as the internal standard. The resultant limit of detection was 1.5 picomoles, requiring a sample size of less than 10 µL of urine. The use of tandem mass spectrometry with a stable isotope labeled internal standard provided high specificity, accuracy, and precision for this assay resulting a recovery of 93.9% and a repeatability standard deviation of 3.78%.
Keyword Creatinine
HILIC
HPLC-MS/MS
Human urine
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: Official 2015 Collection
School of Pharmacy Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in Thomson Reuters Web of Science Article
Scopus Citation Count Cited 0 times in Scopus Article
Google Scholar Search Google Scholar
Created: Tue, 01 Apr 2014, 20:23:09 EST by Charna Kovacevic on behalf of School of Pharmacy