Effects of glucocorticoid exposure on growth and structural maturation of the heart of the preterm piglet

Kim, Min Young, Eiby, Yvonne A., Lumbers, Eugenie R., Wright, Layne L., Gibson, Karen J., Barnett, Amanda C. and Lingwood, Barbara E. (2014) Effects of glucocorticoid exposure on growth and structural maturation of the heart of the preterm piglet. PLoS One, 9 3: e93407.1-e93407.9. doi:10.1371/journal.pone.0093407

Author Kim, Min Young
Eiby, Yvonne A.
Lumbers, Eugenie R.
Wright, Layne L.
Gibson, Karen J.
Barnett, Amanda C.
Lingwood, Barbara E.
Title Effects of glucocorticoid exposure on growth and structural maturation of the heart of the preterm piglet
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2014-03-27
Sub-type Article (original research)
DOI 10.1371/journal.pone.0093407
Open Access Status DOI
Volume 9
Issue 3
Start page e93407.1
End page e93407.9
Total pages 9
Editor Xiongwen Chen
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Language eng
Formatted abstract
Inadequate maintenance of systemic blood flow in neonates following preterm birth is associated with increased morbidity and mortality, and may be due in part to structural immaturity of the myocardium. Maternal glucocorticoid administration is associated with improved cardiovascular function, and possibly promotes structural maturation of the myocardium. This study assessed the structural maturity of the myocardium in male and female preterm and term piglets, and preterm piglets exposed to a regimen of maternal glucocorticoids as used clinically. In preterm, term and glucocorticoid exposed preterm piglets cardiomyocyte maturity was examined by measuring the proportion of binucleated myocytes and the volumes of single living ventricular cardiomyocytes with fluorescence microscopy. Ventricular apoptosis and proliferation were measured by immunohistochemistry. Preterm piglet hearts had fewer binucleated myocytes, smaller myocytes, and more proliferative and fewer apoptotic nuclei than term hearts. Maternal glucocorticoid treatment resulted in increased binucleation with no increase in myocyte volume, and levels of proliferation and apoptosis that were more similar to the term heart. Atrial weights were increased and in female piglets there was an increase in the ratio of left to right ventricular weight. The observed changes in atrial mass and myocyte structural maturation correlated with changes in cardiac function of isolated hearts of littermates. In conclusion, the association between increased myocardial maturation following glucocorticoid exposure, improved cardiac function in littermates, and clinical improvement in human neonatal cardiac function exposed to antenatal glucocorticoids, suggests that glucocorticoid exposure contributes to improved cardiovascular function in preterm infants by promoting myocardial structural maturity.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2015 Collection
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Citation counts: TR Web of Science Citation Count  Cited 9 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 11 times in Scopus Article | Citations
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Created: Fri, 28 Mar 2014, 21:13:11 EST by Dr Barbara Lingwood on behalf of UQ Centre for Clinical Research