Next-generation technologies to determine plastid genome sequences

Henry, Robert J., Rice, Nicole, Edwards, Mark and Nock, Catherine J. (2014). Next-generation technologies to determine plastid genome sequences. In Pal Maliga (Ed.), Chloroplast Biotechnology: Methods and Protocols (pp. 39-46) New York, NY, United States: Humana Press. doi:10.1007/978-1-62703-995-6_2

Author Henry, Robert J.
Rice, Nicole
Edwards, Mark
Nock, Catherine J.
Title of chapter Next-generation technologies to determine plastid genome sequences
Title of book Chloroplast Biotechnology: Methods and Protocols
Place of Publication New York, NY, United States
Publisher Humana Press
Publication Year 2014
Sub-type Research book chapter (original research)
DOI 10.1007/978-1-62703-995-6_2
Series Methods in Molecular Biology
ISBN 9781627039949
ISSN 1064-3745
Editor Pal Maliga
Volume number 1132
Chapter number 2
Start page 39
End page 46
Total pages 8
Total chapters 30
Language eng
Formatted Abstract/Summary
Sequencing of chloroplast genomes is a key tool for analysis of chloroplasts and the impact of manipulation of chloroplast genomes by biotechnology. Advances in genome sequencing allow the complete sequencing of the chloroplast genome and assessment of variation in the chloroplast genome sequences within a plant. Isolation of chloroplast DNA has been a traditional starting point in these analyses, but the capacity of current sequencing technologies allows effective analysis of the chloroplast genome sequence by shotgun sequencing of a preparation of total DNA from the plant. Chloroplast insertions in the nuclear genome can be distinguished by their much lower copy number. Short-read sequences are best assembled by alignment with a reference chloroplast genome.
Keyword DNA sequencing
Genome assembly
Next-generation sequencing
Plastid genome
Q-Index Code B1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Author's prepress title: "Next generation technologies to determine genome sequences".

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Created: Thu, 27 Mar 2014, 00:17:15 EST by Professor Robert Henry on behalf of Qld Alliance for Agriculture and Food Innovation