A suite of sucrose transporters expressed in coats of developing legume seeds includes novel pH-independent facilitators

Zhou, Yuchan, Qu, Hongxia, Dibley, Katherine E., Offler, Christina E. and Patrick, John W. (2007) A suite of sucrose transporters expressed in coats of developing legume seeds includes novel pH-independent facilitators. Plant Journal, 49 4: 750-764. doi:10.1111/j.1365-313X.2006.03000.x


Author Zhou, Yuchan
Qu, Hongxia
Dibley, Katherine E.
Offler, Christina E.
Patrick, John W.
Title A suite of sucrose transporters expressed in coats of developing legume seeds includes novel pH-independent facilitators
Journal name Plant Journal   Check publisher's open access policy
ISSN 0960-7412
1365-313X
Publication date 2007-02-01
Sub-type Article (original research)
DOI 10.1111/j.1365-313X.2006.03000.x
Open Access Status Not yet assessed
Volume 49
Issue 4
Start page 750
End page 764
Total pages 15
Place of publication Chichester, West Sussex, United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract
A suite of newly discovered sucrose transporter genes, PsSUF1, PsSUF4, PvSUT1 and PvSUF1, were isolated from the coats of developing pea (Pisum sativum L.) and bean (Phaseolus vulgaris L.) seeds. Sequence analysis indicated that deduced proteins encoded by PsSUF1, PvSUT1 and PvSUF1 clustered in a separate sub-group under sucrose transporter Clade I, whereas the deduced protein encoded by PsSUF4 clustered in Clade II. When expressed in yeast, these genes were shown to encode sucrose transporters with apparent Michaelis Menten constant (Km) values ranging from 8.9 to 99.8 mm. PvSUT1 exhibited functional characteristics of a sucrose/H+ symporter. In contrast, PsSUF1, PvSUF1 and PsSUF4 supported the pH- and energy independent transport of sucrose that was shown to be bi-directional. These transport properties, together with that of counter transport, indicated that PsSUF1, PvSUF1 and PsSUF4 function as carriers that support the facilitated diffusion of sucrose. Carrier function was unaffected by diethylpyrocarbonate and by maltose competition, suggesting that the sucrose binding sites of these transporters differed from those of known sucrose/H+ symporters. All sucrose transporters were expressed throughout the plant and, of greatest interest, were co-expressed in cells considered responsible for sucrose efflux from seed coats. The possible roles played by the novel facilitators in sucrose efflux from seed coats are discussed.
Keyword Facilitator
Functional characterization
Seed coat
Sucrose transporter
Symporter
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Alliance for Agriculture and Food Innovation
 
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Created: Sat, 22 Mar 2014, 00:59:53 EST by Yuchan Zhou on behalf of Learning and Research Services (UQ Library)