Laterally resolved speciation of arsenic in roots of wheat and rice using fluorescence-XANES imaging

Kopittke, Peter M., de Jonge, Martin D., Wang, Peng, McKenna, Brigid A., Lombi, Enzo, Paterson, David J., Howard, Daryl L., James, Simon A., Spiers, Kathryn M., Ryan, Chris G., Johnson, Alexander A. T. and Menzies, Neal W. (2014) Laterally resolved speciation of arsenic in roots of wheat and rice using fluorescence-XANES imaging. New Phytologist, 201 4: 1251-1262. doi:10.1111/nph.12595


Author Kopittke, Peter M.
de Jonge, Martin D.
Wang, Peng
McKenna, Brigid A.
Lombi, Enzo
Paterson, David J.
Howard, Daryl L.
James, Simon A.
Spiers, Kathryn M.
Ryan, Chris G.
Johnson, Alexander A. T.
Menzies, Neal W.
Title Laterally resolved speciation of arsenic in roots of wheat and rice using fluorescence-XANES imaging
Journal name New Phytologist   Check publisher's open access policy
ISSN 0028-646X
1469-8137
Publication date 2014-01-01
Year available 2013
Sub-type Article (original research)
DOI 10.1111/nph.12595
Open Access Status DOI
Volume 201
Issue 4
Start page 1251
End page 1262
Total pages 12
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell
Language eng
Abstract Accumulation of arsenic (As) within plant tissues represents a human health risk, but there remains much to learn regarding the speciation of As within plants. We developed synchrotron-based fluorescence-X-ray absorption near-edge spectroscopy (fluorescence-XANES) imaging in hydrated and fresh plant tissues to provide laterally resolved data on the in situ speciation of As in roots of wheat (Triticum aestivum) and rice (Oryza sativa) exposed to 2 μM As(V) or As(III). When exposed to As(V), the As was rapidly reduced to As(III) within the root, with As(V) calculated to be present only in the rhizodermis. However, no uncomplexed As(III) was detected in any root tissues, because of the efficient formation of the As(III)-thiol complex - this As species was calculated to account for all of the As in the cortex and stele. The observation that uncomplexed As(III) was below the detection limit in all root tissues explains why the transport of As to the shoots is low, given that uncomplexed As(III) is the major As species transported within the xylem and phloem. Using fluorescence-XANES imaging, we have provided in situ data showing the accumulation and transformation of As within hydrated and fresh root tissues.
Keyword Arsenic toxicity
Fluorescence-XANES imaging
Laterally resolved speciation
Root growth
Speciation
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID FT120100277
DE130100943
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2014 Collection
 
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