Quantitative analysis of intestinal bacterial populations from term infants fed formula supplemented with fructo-oligosaccharides

Xia, Qing, Williams, Timberly, Hustead, Deborah, Price, Pamela, Morrison, Mark and Yu, Zhongtang (2012) Quantitative analysis of intestinal bacterial populations from term infants fed formula supplemented with fructo-oligosaccharides. Journal of Pediatric Gastroenterology and Nutrition, 55 3: 314-320. doi:10.1097/MPG.0b013e3182523254


Author Xia, Qing
Williams, Timberly
Hustead, Deborah
Price, Pamela
Morrison, Mark
Yu, Zhongtang
Title Quantitative analysis of intestinal bacterial populations from term infants fed formula supplemented with fructo-oligosaccharides
Journal name Journal of Pediatric Gastroenterology and Nutrition   Check publisher's open access policy
ISSN 0277-2116
1536-4801
Publication date 2012-01-01
Year available 2012
Sub-type Article (original research)
DOI 10.1097/MPG.0b013e3182523254
Open Access Status Not Open Access
Volume 55
Issue 3
Start page 314
End page 320
Total pages 7
Place of publication Philadelphia, PA United States
Publisher Lippincott Williams and Wilkins
Language eng
Subject 2715 Gastroenterology
2735 Pediatrics, Perinatology, and Child Health
Abstract Objectives: Previous studies of infant formulas supplemented with oligosaccharides reported mixed results regarding the impact on intestinal microbial populations. The objective of this study was to examine the effect of supplementation of an infant formula with fructo-oligosaccharides (FOS) on select groups of intestinal bacteria in term infants. Methods: Four groups of infants were enrolled and fed human milk, a commercially available milk-based infant formula, or infant formula supplemented with 2.0 or 3.0g/L FOS. Dietary intake, stool, and tolerance events were recorded. Fresh stool samples were collected approximately 27 days after feeding the diets (approximately 32 days after birth). Total bacteria, Bacteroides (as commensal bacteria), Bifidobacterium and Lactobacillus, and Clostridium difficile and Escherichia coli were quantified using respective specific real-time PCR assays. Results: The formula feeding groups did not differ in stool consistency and stool frequency or frequency of spit-up or vomit during the entire study. The formula-fed infants tended to have more total bacteria in their stool samples than the human milk-fed infants. The formula-fed infants harbored a greater abundance of C difficile and E coli than the human milk-fed infants, but had a similar abundance of Bacteroides, Bifidobacterium, and Lactobacillus. The FOS supplementation at either dose did not significantly increase the bifidobacterial or lactobacilli populations, or decrease the populations of C difficile, E coli, or Bacteroides. Conclusions: The milk-based formula used in this study supported bifidobacterial and lactobacilli populations comparable with the human milk group; however, this formula did not suppress E coli or C difficile as effectively as human milk.
Keyword Infant formula
Intestinal microbiota
Prebiotics
Realtime PCR
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Diamantina Institute Publications
 
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