The Restriction-Endonuclease Rflfii, Isolated From Ruminococcus-Flavefaciens Fd-1, Recognizes the Sequence 5'-Agtact-3', and Is Inhibited by Site-Specific Adenine Methylation

Morrison, M, Mackie, RI and White, BA (1994) The Restriction-Endonuclease Rflfii, Isolated From Ruminococcus-Flavefaciens Fd-1, Recognizes the Sequence 5'-Agtact-3', and Is Inhibited by Site-Specific Adenine Methylation. Fems Microbiology Letters, 122 1-2: 181-185. doi:10.1111/j.1574-6968.1994.tb07162.x


Author Morrison, M
Mackie, RI
White, BA
Title The Restriction-Endonuclease Rflfii, Isolated From Ruminococcus-Flavefaciens Fd-1, Recognizes the Sequence 5'-Agtact-3', and Is Inhibited by Site-Specific Adenine Methylation
Journal name Fems Microbiology Letters   Check publisher's open access policy
ISSN 0378-1097
Publication date 1994-09-01
Year available 1994
Sub-type Article (original research)
DOI 10.1111/j.1574-6968.1994.tb07162.x
Open Access Status Not yet assessed
Volume 122
Issue 1-2
Start page 181
End page 185
Total pages 5
Place of publication AMSTERDAM
Publisher ELSEVIER SCIENCE BV
Language eng
Abstract Molecular studies of the rumen bacterium Ruminococcus flavefaciens are constrained by the lack of stable gene transfer systems. We report here on the characterization of RflFII, a restriction endonuclease isolated from R. flavefaciens FD-1. The enzyme is an isoschizomer of ScaI, and cleavage of the DNA is blunt-ended, between the internal TA dinucleotide sequence of 5'-AGTACT-3'. Chromosomal DNA preparations were used to demonstrate that adenine methylation of DNA within the sequence 5'-GTAC-3' inhibits both RflFII and the restriction endonucleases RsaI and ScaI. Chromosomal DNA from R. flavefaciens FD-1 is also host modified to protect against cleavage by ScaI.
Keyword Restriction Enzyme
Rumen Bacteria
Ruminococcus Flavefaciens
Albus
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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