Partial-Purification and Characterization of Ral8I, a Class-Iis Restriction Endonuclease From Ruminococcus-Albus-8 Which Recognizes 5'-Ggatc

Morrison, M, Mackie, RI and White, BA (1992) Partial-Purification and Characterization of Ral8I, a Class-Iis Restriction Endonuclease From Ruminococcus-Albus-8 Which Recognizes 5'-Ggatc. Gene, 111 1: 105-108. doi:10.1016/0378-1119(92)90609-S


Author Morrison, M
Mackie, RI
White, BA
Title Partial-Purification and Characterization of Ral8I, a Class-Iis Restriction Endonuclease From Ruminococcus-Albus-8 Which Recognizes 5'-Ggatc
Journal name Gene   Check publisher's open access policy
ISSN 0378-1119
Publication date 1992-02-01
Year available 1992
Sub-type Other
DOI 10.1016/0378-1119(92)90609-S
Open Access Status Not yet assessed
Volume 111
Issue 1
Start page 105
End page 108
Total pages 4
Place of publication AMSTERDAM
Publisher ELSEVIER SCIENCE BV
Language eng
Abstract Heparin-agarose chromatography was used to isolate a restriction endonuclease (ENase) from the cellulolytic Gram+ anaerobe, Ruminococcus albus 8. The enzyme, Ral8I, was eluted from the column using 230-310 mM Na+. However, the preparation was active only with DNA substrates that were not Dam-methylated. Moreover, the restriction fragment pattern generated from-simian virus 40 (SV40) DNA was not consistent with the expected number of Dam-methylation sites. Alignment of the Dam-methylation sites in SV40 DNA indicated that Ral8I may actually recognize the asymmetric sequence, GGATC. This was confirmed by nucleotide (nt) sequence analysis and, further, Ral8I was found to cause cleavage of the DNA approx. 5 nt downstream from the recognition sequence. Ral8I can therefore be classified as a type-IIS restriction endonuclease and is an isoschizomer of AlwI, BinI and BthII.
Keyword Recombinant Dna
Bini Isoschizomer
Gram+ Anaerobe
Type-Iis Enzyme
Inhibition by Dam Methylation
Plasmid Dna
Electroporation
Q-Index Code CX
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Other
Collection: ResearcherID Downloads - Archived
 
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